药物流产后蜕膜组织中IL-10和IL-12表达水平及其对异常子宫出血的影响

doi:10.13481/j.1671-587x.20160127

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (01): 134-138.doi: 10.13481/j.1671-587x.20160127

药物流产后蜕膜组织中IL-10和IL-12表达水平及其对异常子宫出血的影响

魏静波¹, 刘辉², 朱小茼³, 刘聪慧⁴, 曲银娥¹

1. 华北理工大学组织胚胎学系, 河北唐山 063000;
2. 华北理工大学办公室, 河北唐山 063000;
3. 华北理工大学心理学院, 河北唐山 063000;
4. 华北理工大学附属医院妇产科, 河北唐山 063000

收稿日期:2015-01-29 发布日期:2016-01-26
通讯作者: 曲银娥,教授,硕士研究生导师(Tel:0315-3725754,E-mail:linlin0852@sina.com) E-mail:linlin0852@sina.com
作者简介:魏静波(1978-),女,内蒙古自治区赤峰市人,讲师,医学硕士,主要从事生殖免疫和肿瘤免疫方面的研究。
基金资助:
河北省科技厅科研基金资助课题(12277747);河北省唐山市科技局科研基金资助课题(12150222B-16)

Expression levels of IL-10 and IL-12 in decidua tissue and their influence in abnormal uterine bleeding after abortion

WEI Jinbo¹, LIU Hui², ZHU Xiaotong³, LIU Conghui⁴, QU Yine¹

1. Department of Histology and Embryology, North China University of Science and Technology, Tangshan 063000, China;
2. Office of North China University of Science and Technology, Tangshan 063000, China;
3. School of Psychology, North China University of Science and Technology, Tangshan 063000, China;
4. Department of Obstetrics and Gynecology, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, China

Received:2015-01-29 Published:2016-01-26

摘要/Abstract

摘要：

目的: 观察白细胞介素10(IL-10)和白细胞介素12(IL-12)在药物流产后蜕膜组织中的表达,探讨其表达水平及其与药物流产后异常子宫出血的关系。方法: 选取自愿终止妊娠的停经8周之内的早孕妇女,自愿选择药物流产(药流)或人工流产2种方法,分为人工流产组、药流不全组和药流完全组,每组20例。应用免疫组织化学法和Western blotting法检测IL-10和IL-12在各组蜕膜组织原位的表达情况。结果: 免疫组织化学法,IL-10和IL-12阳性细胞主要分布在子宫蜕膜上皮和基质细胞之间。药流组蜕膜组织IL-10表达水平低于人工流产组(P<0.01),且药流完全组IL-10表达水平低于药流不全组(P<0.01);药流组蜕膜组织IL-12表达水平高于人工流产组(P<0.01),且药流完全组IL-12表达水平高于药流不全组(P<0.01)。Western blotting法检测结果与免疫组织化学方法检测结果吻合。结论: 局部蜕膜组织对米非司酮作用不敏感,导致IL-10和IL-12表达水平失衡,二者对药物流产后异常子宫出血的发生具有促进作用。

关键词: 子宫出血, 白细胞介素10, 白细胞介素12, 免疫应答, 免疫耐受

Abstract:

Objective: To investigate the expressions of IL-10 and IL-12 in deciduas tissue of the women after abortion,to explore the correlation between the expressions levels of IL-10 and IL-12 in decidua tissue and abnormal uterine bleeding after abortion. Methods: The healthy pregnant women required termination with gestational age less than 8 weeks were recruited.The methods of drug abortion or surgical abortion were selected according to the women's own will.The women were divided into surgical abortion by vaccum aspiration group,incomplete mifepristone abortion group and complete mifepristone abortion group(n=20).The immunohistochemistry method and Western blotting method were used to detect the exoressions of IL-10 and IL-12 in decidua tissue of the women in various groups.Results: The results of immunohistochemistry method showed that the positive cells of IL-10 and IL-12 scattered among epithelium,glands and stromal cells.The IL-10 expression levels in decidua tissue in mifepristone abortion groups were significantly lower than that in surgical abortion group (P<0.01),and the IL-10 expression level in complete mifepristone abortion group was significantly lower than that in incomplete mifepristone abortion group(P<0.01).The IL-12 expression levels in decidua tissue in mifepristone abortion groups were significantly higher than that in surgical abortion group (P<0.01),and the IL-12 expression level in complete mifepristone abortion group was significantly lower than that in incomplete mifepristone abortion group(P<0.01).The results of Western blotting method were coincided with the results of immunohistochemistry method. Conclusion: Local decidua tissue is not sensitive to mifepristone,which causes the imbalance of expressions of IL-10 and IL-12,and they can promote the abnormal uterine bleeding after abortion.

Key words: uterine bleeding, interleukin-10, interleukin-12, immune response, immune tolerance

中图分类号:

R364.7

魏静波, 刘辉, 朱小茼, 刘聪慧, 曲银娥. 药物流产后蜕膜组织中IL-10和IL-12表达水平及其对异常子宫出血的影响[J]. 吉林大学学报(医学版), 2016, 42(01): 134-138.

WEI Jinbo, LIU Hui, ZHU Xiaotong, LIU Conghui, QU Yine. Expression levels of IL-10 and IL-12 in decidua tissue and their influence in abnormal uterine bleeding after abortion[J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 134-138.

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药物流产后蜕膜组织中IL-10和IL-12表达水平及其对异常子宫出血的影响

Expression levels of IL-10 and IL-12 in decidua tissue and their influence in abnormal uterine bleeding after abortion

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