吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (04): 759-763.doi: 10.13481/j.1671-587x.20180412

• 基础研究 • 上一篇    下一篇

菩人丹超微粉对STZ诱导糖尿病大鼠视网膜病变的保护作用及其对NF-κB信号通路的影响

王海彬, 董志军, 郭立涛, 石晶, 董微丽   

  1. 承德医学院附属医院眼科, 河北 承德 067000
  • 收稿日期:2017-12-07 出版日期:2018-07-28 发布日期:2018-07-27
  • 通讯作者: 董微丽,教授,主任医师,硕士研究生导师(Tel:0314-2279536,E-mail:healthymybody@126.com) E-mail:healthymybody@126.com
  • 作者简介:王海彬(1975-),男,河北省承德市人,副教授,副主任医师,医学硕士,主要从事眼底病和泪器疾病方面的研究。
  • 基金资助:
    河北省中医药管理局科研基金资助课题(2014065)

Protective effect of Purendan Superfine Powder on retinopathy of rats with diabetes mellitus induced by streptozotocin and its influence in NF-κB signaling pathway

WANG Haibin, DONG Zhijun, GUO Litao, SHI Jing, DONG Weili   

  1. Department of Ophthalmology, Affiliated Hospital, Chengde Medical College, Chengde 067000, China
  • Received:2017-12-07 Online:2018-07-28 Published:2018-07-27

摘要: 目的:探讨菩人丹超微粉(PRD)对糖尿病大鼠视网膜的保护作用及其对核因子κB (NF-κB)信号通路的影响,并阐明其作用机制。方法:40只Wistar大鼠随机分为正常对照组、模型组、低剂量PRD组和高剂量PRD组。给药3个月后,测定各组大鼠血糖水平,RT-PCR法检测大鼠视网膜组织中血管内皮生长因子(VEGF)和血管生成素2(Ang-2) mRNA表达水平,Western blotting法检测大鼠视网膜组织中晚期糖基化终末产物(AGEs)、晚期糖基化终末产物受体(RAGE)、NF-κB、VEGF和Ang-2蛋白表达水平。结果:与正常对照组比较,模型组大鼠血糖水平及视网膜组织中VEGF、Ang-2 mRNA表达水平和AGEs、RAGE、NF-κB、VEGF、Ang-2蛋白表达水平明显升高(P<0.05);与模型组比较,低和高剂量PRD组大鼠血糖水平及视网膜组织中VEGF、Ang-2 mRNA表达水平和AGEs、RAGE、NF-κB、VEGF、Ang-2蛋白表达水平明显降低(P<0.05)。结论:PRD可能通过特异性阻断AGEs/RAGE/NF-κB信号通路对糖尿病大鼠视网膜起保护作用。

关键词: 实验性, 核因子κB信号通路, 视网膜病变, 菩人丹超微粉, 糖尿病

Abstract: Objective:To investigate the protective effect of Purendan superfine powder (PRD) on the retinopathy of the diabetic rats and its influence in the nuclear factor kappa B (NF-κB) signaling pathway,and to elucidate its mechanism of action. Methods: Forty Wistar rats were randomly divided into normal control group,model group,low dose of PRD group and high dose of PRD group.After 3 months of administration,the blood sugar levels of the rats in various groups were detected.The expression levels of vascular endothelial growth factor(VEGF) and angiogenin-2(Ang-2) mRNA in retina tissue of the rats were determined by RT-PCR.The expression levels of advanced glycation end products(AGEs),receptor for advanced glycation end products (RAGE),NF-κB,VEGF,Ang-2 proteins in retina tissue of the rats were determined by Western blotting method. Results:Compared with normal control group,the blood sugar level,the expression levels of VEGF,Ang-2 mRNA in retina tissue and the expression levels of AGEs,RAGE,NF-κB,VEGF,Ang-2 proteins in retina of the rats in model group were significantly increased(P<0.05).Compared with model group,the blood sugar levels,the expression levels of VEGF and Ang-2 mRNA in retina tissue,and the expression levels of AGEs,RAGE,NF-κB,VEGF,Ang-2 proteins in retina tissue of the rats in low and high doses of PRD group were significantly decreased(P<0.05). Conclusion: PRD may protect the retina of diabetic rats by specifically blocking the AGEs/RAGE/NF-κB signaling pathway.

Key words: diabetes mellitus,experimental, retinopathy, Purendan Superfine Powder, nuclear factor kappa B signaling pathway

中图分类号: 

  • R285.5