菩人丹超微粉对STZ诱导糖尿病大鼠视网膜病变的保护作用及其对NF-κB信号通路的影响

doi:10.13481/j.1671-587x.20180412

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (04): 759-763.doi: 10.13481/j.1671-587x.20180412

菩人丹超微粉对STZ诱导糖尿病大鼠视网膜病变的保护作用及其对NF-κB信号通路的影响

王海彬, 董志军, 郭立涛, 石晶, 董微丽

承德医学院附属医院眼科, 河北承德 067000

收稿日期:2017-12-07 出版日期:2018-07-28 发布日期:2018-07-27
通讯作者: 董微丽,教授,主任医师,硕士研究生导师(Tel:0314-2279536,E-mail:healthymybody@126.com) E-mail:healthymybody@126.com
作者简介:王海彬(1975-),男,河北省承德市人,副教授,副主任医师,医学硕士,主要从事眼底病和泪器疾病方面的研究。
基金资助:
河北省中医药管理局科研基金资助课题（2014065）

Protective effect of Purendan Superfine Powder on retinopathy of rats with diabetes mellitus induced by streptozotocin and its influence in NF-κB signaling pathway

WANG Haibin, DONG Zhijun, GUO Litao, SHI Jing, DONG Weili

Department of Ophthalmology, Affiliated Hospital, Chengde Medical College, Chengde 067000, China

Received:2017-12-07 Online:2018-07-28 Published:2018-07-27

摘要/Abstract

摘要： 目的：探讨菩人丹超微粉（PRD）对糖尿病大鼠视网膜的保护作用及其对核因子κB （NF-κB）信号通路的影响，并阐明其作用机制。方法：40只Wistar大鼠随机分为正常对照组、模型组、低剂量PRD组和高剂量PRD组。给药3个月后，测定各组大鼠血糖水平，RT-PCR法检测大鼠视网膜组织中血管内皮生长因子（VEGF）和血管生成素2（Ang-2） mRNA表达水平，Western blotting法检测大鼠视网膜组织中晚期糖基化终末产物（AGEs）、晚期糖基化终末产物受体（RAGE）、NF-κB、VEGF和Ang-2蛋白表达水平。结果：与正常对照组比较，模型组大鼠血糖水平及视网膜组织中VEGF、Ang-2 mRNA表达水平和AGEs、RAGE、NF-κB、VEGF、Ang-2蛋白表达水平明显升高（P<0.05）；与模型组比较，低和高剂量PRD组大鼠血糖水平及视网膜组织中VEGF、Ang-2 mRNA表达水平和AGEs、RAGE、NF-κB、VEGF、Ang-2蛋白表达水平明显降低（P<0.05）。结论：PRD可能通过特异性阻断AGEs/RAGE/NF-κB信号通路对糖尿病大鼠视网膜起保护作用。

关键词: 实验性, 核因子κB信号通路, 视网膜病变, 菩人丹超微粉, 糖尿病

Abstract: Objective:To investigate the protective effect of Purendan superfine powder (PRD) on the retinopathy of the diabetic rats and its influence in the nuclear factor kappa B (NF-κB) signaling pathway,and to elucidate its mechanism of action. Methods: Forty Wistar rats were randomly divided into normal control group,model group,low dose of PRD group and high dose of PRD group.After 3 months of administration,the blood sugar levels of the rats in various groups were detected.The expression levels of vascular endothelial growth factor(VEGF) and angiogenin-2(Ang-2) mRNA in retina tissue of the rats were determined by RT-PCR.The expression levels of advanced glycation end products(AGEs),receptor for advanced glycation end products (RAGE),NF-κB,VEGF,Ang-2 proteins in retina tissue of the rats were determined by Western blotting method. Results:Compared with normal control group,the blood sugar level,the expression levels of VEGF,Ang-2 mRNA in retina tissue and the expression levels of AGEs,RAGE,NF-κB,VEGF,Ang-2 proteins in retina of the rats in model group were significantly increased(P<0.05).Compared with model group,the blood sugar levels,the expression levels of VEGF and Ang-2 mRNA in retina tissue,and the expression levels of AGEs,RAGE,NF-κB,VEGF,Ang-2 proteins in retina tissue of the rats in low and high doses of PRD group were significantly decreased(P<0.05). Conclusion: PRD may protect the retina of diabetic rats by specifically blocking the AGEs/RAGE/NF-κB signaling pathway.

Key words: diabetes mellitus,experimental, retinopathy, Purendan Superfine Powder, nuclear factor kappa B signaling pathway

中图分类号:

R285.5

王海彬, 董志军, 郭立涛, 石晶, 董微丽. 菩人丹超微粉对STZ诱导糖尿病大鼠视网膜病变的保护作用及其对NF-κB信号通路的影响[J]. 吉林大学学报(医学版), 2018, 44(04): 759-763.

WANG Haibin, DONG Zhijun, GUO Litao, SHI Jing, DONG Weili. Protective effect of Purendan Superfine Powder on retinopathy of rats with diabetes mellitus induced by streptozotocin and its influence in NF-κB signaling pathway[J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 759-763.

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菩人丹超微粉对STZ诱导糖尿病大鼠视网膜病变的保护作用及其对NF-κB信号通路的影响

Protective effect of Purendan Superfine Powder on retinopathy of rats with diabetes mellitus induced by streptozotocin and its influence in NF-κB signaling pathway

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