吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (04): 791-795.doi: 10.13481/j.1671-587x.20180418

• 临床研究 • 上一篇    下一篇

米非司酮对稽留流产患者血清和绒毛组织中Ang-1、Ang-2、VEGF、TNF-α和IL-6表达水平的影响

郑燕, 王娜, 吴命坤, 刘静乔, 孟亚丽   

  1. 河北医科大学第一医院妇科, 河北 石家庄 050031
  • 收稿日期:2017-10-26 出版日期:2018-07-28 发布日期:2018-07-27
  • 通讯作者: 孟亚丽,主任医师,硕士研究生导师(Tel:0311-85917086,E-mail:yanzidongnanfei9@163.com) E-mail:yanzidongnanfei9@163.com
  • 作者简介:郑燕(1969-),女,河南省温县人,副主任医师,主要从事稽留流产方面的研究。
  • 基金资助:
    河北省卫计委科研项目资助课题(20160709)

Influence of mifepristone in expression levels of Ang-1,Ang-2, VEGF, TNF-α and IL-6 in serum and villus tissue of patients with missed abortion

ZHENG Yan, WANG Na, WU Mingkun, LIU Jingqiao, MENG Yali   

  1. Department of Gynaecology and Obstetrics, First Hospital, Hebei Medical University, Shijiazhuang 050031, China
  • Received:2017-10-26 Online:2018-07-28 Published:2018-07-27

摘要: 目的:检测稽留流产患者服用米非司酮前后血清和绒毛组织中血管生成素1(Ang-1)、血管生成素2(Ang-2)、血管内皮生长因子(VEGF)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)表达水平,阐明米非司酮对稽留流产的作用机制。方法:选取确诊为稽留流产患者68例作为药流组,另选取同期自愿要求终止妊娠的正常孕妇51例作为对照组。应用酶联免疫吸附法检测2组研究对象引产前后血清中Ang-1、Ang-2、VEGF、TNF-α和IL-6水平,采用免疫组织化学染色法检测绒毛组织中Ang-1、Ang-2、VEGF、TNF-α和IL-6表达水平。结果:与引产前比较,药流组患者引产后血清中Ang-1、Ang-2、VEGF、TNF-α和IL-6水平明显升高(P<0.05)。与对照组比较,药流组患者引产后绒毛组织中Ang-1、Ang-2、VEGF和TNF-α表达水平明显升高(P<0.05),IL-6表达水平明显降低(P<0.05)。药流组患者血清和绒毛组织中Ang-1、Ang-2与VEGF水平呈正相关关系(r=0.849,r=0.721,r=0.590,r=0.532,P<0.05)。结论:米非司酮通过提高稽留流产患者血清和绒毛组织中Ang-1、Ang-2、VEGF和TNF-α水平,降低IL-6水平,促进血管生成,对终止稽留流产起促进作用。

关键词: 血管内皮生长因子, 血管生成素2, 白细胞介素6, 血管生成素1, 米非司酮, 肿瘤坏死因子α

Abstract: Objective:To detect the expression levels of angiogenin-1, 2 (Ang-1, 2), vascular endothelial growth factor (VEGF), tumour necrosis factor (TNF-α) and interleukin-6 (IL-6) in the serum and villus tissue of the patients with missed abortion before and after taking mifepristone,and to elucidate the mechanism of mifepristone for the treatment of missed abortion. Methods: A total of 68 cases of patients with missed abortion were selected as drug abortion group,and 51 cases of healthy pregnant women were selected as control group. ELISA was applied to detect the levels of Ang-1, Ang-2, VEGF, TNF-α, and IL-6 in serum of the patients; immunohistochemistry was used to detect the expression levels of Ang-1, Ang-2, VEGF, TNF-α, and IL-6 in villus tissue. Results:The levels of Ang-1, Ang-2, VEGF, TNF-α and IL-6 in serum of the patients in drug abortion group after abortion were higher than those before abortion(P<0.05).The expression levels of Ang-1, Ang-2, VEGF, and TNF-α in drug abortion group after abortion were higher than those in control group(P<0.05), but the IL-6 expression level was lower than that in control group (P<0.05). The levels of Ang-1 and Ang-2 in serum and villus tissue had positive correlation with the VEGF level(r=0.849,r=0.721,r=0.590,r=0.532,P<0.05). Conclusion:Mifepristone can promote the termination of missed abortion by increasing the levels of Ang-1, Ang-2, VEGF, and TNF-α in serum and villus tissue and decreasing the IL-6 level.

Key words: angiopoietin 1, interleukin-6, mifepristone, angiopoietin 2, vascular endothelial growth factor, tumor necrosis factor-α

中图分类号: 

  • R735.7