关键词: 神经胶质瘤细胞, 增殖细胞核抗原, 基因表达

Abstract: To determine the effect of heroin on the gene expression of proliferating cell nuclear antigen(PCNA) in rat C6 glioma cells，and investigate the molecular mechanism of heroin addiction.Methods The rat C6 glioma cells were treated with heroin with doses of 0－100 mg•L^-1 for 24 h,MTT assay was used to detect the viability of cells,the gene expression level of PCNA was determined by RT-PCR. Results When the C6 cells were treated with heroin with doses of 10,20 and 100 mg•L^-1,the cell viabilities were 90.62%，80.97%,and 62.73%,there were significant differences compared with control group(P<0.05).When treated with 20 mg•L^-1 heroin for 48 h,the expression level of PCNA mRNA of C6 glioma cells was decreased as compared with corresponding control cells,the ratio was 0.297（P<0.01）.Conclusion Heroin with dose of 20 mg•L^-1 can inhibit the growth of C6 glioma cells by 30 percent.It may be related to heroin addiction.

Key words: glioma cells, proliferating cell nuclear antigen, gene expression