吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 554-558.doi: 10.7694/jldxyxb20130327

• 基础研究 • 上一篇    下一篇

SHIP和Caspase-3 mRNA在骨髓增生异常综合征患者骨髓组织中的表达及其临床意义

贾晓辉1,罗建民2,刘亚平1,尹婉宜1,张丽红1   

  1. 1.河北医科大学第一医院血液内科,河北 石家庄 050031;2. 河北医科大学第二医院血液内科,河北 石家庄 050000
  • 收稿日期:2013-02-04 出版日期:2013-05-28 发布日期:2013-07-01
  • 通讯作者: 罗建民(Tel:0311-66003927,E-mail:luojm315@yahoo.com.cn) E-mail:luojm315@yahoo.com.cn
  • 作者简介:贾晓辉(1970-),男,河北省武强县人,主治医师,医学硕士,主要从事恶性血液病的基础与临床研究。
  • 基金资助:

    河北省自然科学基金资助课题(H2012206139)

Expressions of SHIP and Caspase-3 mRNA in bone marrow tissue of

JIA Xiao-hui1,LUO Jian-min2,LIU Ya-ping1,YIN Wan-yi1,ZHANG LI-hong1   

  1. 1.Department of Hematology,First Hospital,Hebei Medical University,Shijiazhuang 050031,China; 2. Department of Hematology,Second Hospital,Hebei Medical University,Shijiazhuang 050000,China
  • Received:2013-02-04 Online:2013-05-28 Published:2013-07-01

摘要: [摘 要] 目的:检测骨髓增生异常综合征(MDS)患者骨髓组织中SHIP、Caspase-3 mRNA的表达,探讨其表达与MDS发病及向白血病转化过程的关系。方法:根据国际预后评分系统(IPSS)选择62例MDS患者,其中低危(low-risk)组20例、中危1(Int-1)组12例、中危2(Int-2)组13例和高危(high-risk)组17例,采用RT-PCR法检测62例MDS患者及30例健康正常者(对照组)骨髓单个核细胞(BMNC)中SHIP mRNA及Caspase-3 mRNA的表达水平,并分析两者之间的关系。 结果:MDS低危组和中危1组BMNC中SHIP mRNA表达率(100%,100%)及表达水平(4.467±2.899,4.529±1.975)分别与对照组(100%,4.623±2.979)比较差异均无统计学意义(P>0.05);在中危2组和高危组中,BMNC中SHIP表达率(92%,88%)下降,表达水平(2.124±1.880,2.574±1.807)低于对照组(P<0.05)。Pearson相关分析,MDS患者BMNC中SHIP mRNA与Caspase-3 mRNA表达水平呈正相关关系(r=0.714,P<0.05)。随骨髓原始细胞比例升高,SHIP mRNA表达水平降低(F=3.7,P<0.05) ,不同性别(t=0.940,P>0.05)、不同年龄(t=1.419,P>0.05)患者SHIP mRNA表达水平差异无统计学意义。 结论:SHIP在高危MDS患者BMNC中呈低表达或不表达,其表达水平可以作为判定MDS预后的一个指标。

关键词: 骨髓增生异常综合征, 基因表达, SHIP, 聚合酶链反应

Abstract: Abstract:Objective To detect the expressions of SHIP mRNA and Caspase-3 mRNA in bone marrow tissue of  the patients with   myelodysplastic syndrome (MDS) and to discuss the relationship between SHIP mRNA and Caspase-3 mRNA expressions and the occurrence and development of MDS.Methods 20 low-risk MDS cases(low-risk group),12 Int-1 MDS cases(Int-1 group),13 Int-2 MDS cases(Int-2 group),and 17 high-risk MDS cases(high-risk group) were selected according to International Prognostic Scoring Syterm(IPSS).30 healthy volunteers were regared as control group.The expression levels of SHIP mRNA and Caspase-3 mRNA in bone marrow mononuclear  cells (BMNC) in various groups were detected by RT-PCR method,and the relationship between them was analyzed. Results The positive rates of SHIP mRNA were 100% and 100% and its expression levels were 4.467±2.899 and 4.529±1.975 in low-risk group and Int-1 group,respectively,while they were 100% and 4.623±2.979 in control group,so there were no significant differences between low-risk group,Int-1 group and control group (P>0.05).The positive rates of SHIP mRNA were 90% and 88% and the expression levels were 2.124±1.880 and 2.574±1.807 in Int-2 group and high-risk group,respectively,so there were significantly differences compared with control group (P<0.05).The Pearson correlation analysis results showed that the positive correlations were found between the level of SHIP mRNA and the level of Caspase-3 mRNA in MDS patients(r=0.714,P>0.05).The expression level of SHIP mRNA was decreased with the increasing of premitive cells(F=3.7,P<0.05).There were no significant differences of SHIP mRNA expression levels of patients between different genders(t=0.940,P>0.05) and different ages(t=1.417,P>0.05).Conclusion The expression level of SHIP  is lower or the SHIP doesn’t  express in  BMNC of the high-risk MDS patients, and  its expression level   can be used as an indicator to judge the prognosis of MDS.

Key words: myelodysplastic syndrome, gene expression, SH2 domain-containing inositol 5&rsquo, -phosphatase, polymerase chain reactiom

中图分类号: 

  • R551.3