吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (02): 281-285.doi: 10.13481/j.1671-587x.20180214

• 基础研究 • 上一篇    下一篇

室内装修挥发性污染物对雄性小鼠生殖功能和精子凋亡的影响

朱博1,2, 满枋霖1, 刘鹏1, 佟海滨1, 田丹1, 孙新1   

  1. 1. 北华大学生命科学研究中心, 吉林 吉林 132013;
    2. 北华大学附属医院生殖医学中心, 吉林 吉林 132011
  • 收稿日期:2017-09-01 出版日期:2018-03-28 发布日期:2018-03-30
  • 通讯作者: 孙新,教授,主任,硕士研究生导师(Tel:0432-64608351,E-mail:sunxinbh@126.com);田丹,助理研究员(Tel:0432-64608350,E-mail:tiantiande2004@163.com) E-mail:sunxinbh@126.com;tiantiande2004@163.com
  • 作者简介:朱博(1989-),男,吉林省吉林市人,医师,医学硕士,主要从事生殖医学方面的研究。
  • 基金资助:
    吉林省卫计委科研计划项目资助课题(2013Z069);吉林省科技厅科技计划项目资助课题(20150312013ZG)

Influence of indoor decoration volatile pollutants in reproductive function and sperm apoptosis of male mice

ZHU Bo1,2, MAN Fanglin1, LIU Peng1, TONG Haibin1, TIAN Dan1, SUN Xin1   

  1. 1. Life Science Research Center, Beihua University, Jilin 132013, China;
    2. Reproduction Medicine Center, Affiliated Hospital, Beihua University, Jilin 132013, China
  • Received:2017-09-01 Online:2018-03-28 Published:2018-03-30

摘要: 目的:模拟室内装修主要挥发性物质的污染环境,探讨室内装修主要挥发性污染物甲醛、苯系物及氨对雄性小鼠生殖功能的影响。方法:选取雄性ICR小鼠20只,随机平均分为实验组和对照组,实验组小鼠进行甲醛(60 mg·m-3)、二甲苯(50 mg·m-3)和氨(40 mg·m-3)经呼吸道混配静式染毒,同时对照组小鼠放入空气填充的静式染毒柜中。每天固定时间称量小鼠体质量并记录,连续染毒35d。染毒期间每天观察小鼠行为学变化。收集小鼠附睾尾部精子并计数,检测精子密度及活力;采用Diff-Quick染色法染色,计算精子畸形率;采用流式细胞术(FCM)检测小鼠附睾尾部精子凋亡率;采用Western blotting法检测小鼠睾丸组织中细胞凋亡相关因子Caspase-9和Cleaved Caspase-3蛋白表达水平。结果:染毒28d后,实验组小鼠体质量与对照组比较明显降低(P<0.01);染毒35d后,实验组小鼠精子浓度、活力及精子畸形率与对照组比较均明显下降(P<0.01);与对照组比较,实验组小鼠精子凋亡率明显升高(P<0.01)。实验组与对照组小鼠睾丸组织中Caspase-9和Cleaved Caspase-3蛋白表达水平比较差异无统计学意义(P>0.05)。结论:室内装修主要污染物能够明显影响雄鼠附睾中精子质量。

关键词: 精子, 细胞凋亡, 甲醛, 二甲苯, 氨, 男性不育

Abstract: Objective:To simulate the pollution environment of indoor decoration volatile pollutants,and to explore the influence of three main indoor decoration volatile pollutants formaldehyde,xylene and ammonia in the male mouse reproductive function. Methods: A total of 20 male ICR mice were randomly divided into experimental group (exposed to 60 mg·m-3 formaldehyde,50 mg·m-3 xylene and 40 mg·m-3 ammonia mixed in the static type canister) and control group (put into the air-filled static type canister) (n=10).Each mouse was weighed at set times every day,exposed for 35 d continuously.The behavior changes of the mice were also observed everyday.The epididymal cauda sperms were collected to detect the sperm density and vitality.The sperm malformation rate was detected by Diff-Quick dyeing method.The apoptotic rate of epididymal cauda sperms was detected by flow cytometry(FCM).Then Western blotting method was used to determine the expression levels of Caspase-9 and Cleaved Caspase-3 proteins in testis tissue of the mice. Results: Four weeks after the exposure to pollutants,the body weight of the mice in experimental group was significantly lower than that in control group (P<0.01).The concentration and vitality of sperm malformation rate of the mice in experimental group were significantly lower than those in control group (P<0.01) 35 d after exposure.Compared with control group, the apoptotic rate of sperm of the mice in experimental group was significantly increased (P<0.01).However,the expression levels of Caspase-9 and Cleaved Caspase-3 in the testis tissue of the mice in experimental group and control group had no significant differences (P>0.05). Conclusion: The main pollutants in indoor decoration can significantly influence the quality of spermatozoa in the epididymis.

Key words: formaldehyde, sperm, xylene, ammonia, apoptosis, male infertility

中图分类号: 

  • R697.22