关键词: 精子, 细胞凋亡, 甲醛, 二甲苯, 氨, 男性不育

Abstract: Objective:To simulate the pollution environment of indoor decoration volatile pollutants,and to explore the influence of three main indoor decoration volatile pollutants formaldehyde,xylene and ammonia in the male mouse reproductive function. Methods: A total of 20 male ICR mice were randomly divided into experimental group (exposed to 60 mg·m^-3 formaldehyde,50 mg·m^-3 xylene and 40 mg·m^-3 ammonia mixed in the static type canister) and control group (put into the air-filled static type canister) (n=10).Each mouse was weighed at set times every day,exposed for 35 d continuously.The behavior changes of the mice were also observed everyday.The epididymal cauda sperms were collected to detect the sperm density and vitality.The sperm malformation rate was detected by Diff-Quick dyeing method.The apoptotic rate of epididymal cauda sperms was detected by flow cytometry(FCM).Then Western blotting method was used to determine the expression levels of Caspase-9 and Cleaved Caspase-3 proteins in testis tissue of the mice. Results: Four weeks after the exposure to pollutants,the body weight of the mice in experimental group was significantly lower than that in control group (P<0.01).The concentration and vitality of sperm malformation rate of the mice in experimental group were significantly lower than those in control group (P<0.01) 35 d after exposure.Compared with control group, the apoptotic rate of sperm of the mice in experimental group was significantly increased (P<0.01).However,the expression levels of Caspase-9 and Cleaved Caspase-3 in the testis tissue of the mice in experimental group and control group had no significant differences (P>0.05). Conclusion: The main pollutants in indoor decoration can significantly influence the quality of spermatozoa in the epididymis.

Key words: formaldehyde, sperm, xylene, ammonia, apoptosis, male infertility