磷酸化胞外信号调节激酶在小鼠海马发育过程中的表达及其意义

doi:10.13481/j.1671-587x.20180516

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (05): 979-982.doi: 10.13481/j.1671-587x.20180516

磷酸化胞外信号调节激酶在小鼠海马发育过程中的表达及其意义

王天月¹, 崔晓燕², 吴骁³, 王丹⁴

1. 锦州医科大学附属第三医院血液科, 辽宁锦州 121001;
2. 河北省药品检验研究院药理毒理室, 河北石家庄 050011;
3. 辽宁何氏医学院实验动物中心, 辽宁沈阳 110163;
4. 锦州医科大学人体解剖与组织胚胎学教研室, 辽宁锦州 121001

收稿日期:2017-11-28 出版日期:2018-09-28 发布日期:2018-11-20
通讯作者: 王丹,讲师,硕士研究生导师(Tel:0416-3999207,E-mail:caoyanju2000@163.com) E-mail:caoyanju2000@163.com
作者简介:王天月(1974-),男,辽宁省锦州市人,主治医师,医学硕士,主要从事神经发育及血液肿瘤方面的研究。
基金资助:
辽宁省科技厅发展计划处自然科学基金项目资助课题（2015020696）

Expression of phosphorylated extracellular signal-regulated kinase during development of hippocampus in mice

WANG Tianyue¹, CUI Xiaoyan², WU Xiao³, WANG Dan⁴

1. Department of Hematology, Third Affiliated Hospital, Jinzhou Medical University, Jinzhou 121001, China;
2. Pharmacology and Toxiology Office, Hebei Institute for Pharmaceutical Testing, Shijiazhuang 050011, China;
3. Experimental Animal Center, Liaoning Ho's Medical College, Shenyang 110163, China;
4. Department of Human Anatomy, Histology and Embryology, Jinzhou Medical University, Jinzhou 121001, China

Received:2017-11-28 Online:2018-09-28 Published:2018-11-20

摘要/Abstract

摘要： 目的：观察磷酸化胞外信号调节激酶（pERK）在胚龄（E）18 d以及生后不同日龄（P）小鼠海马不同阶段的表达，探讨其在海马发育中的可能作用。方法：分别取E18 d和P 1、3、7、14、21和28 d小鼠的海马组织，每个时间点均作为观察组，采用免疫组织化学和免疫印迹法、结合图像分析技术检测各时间点海马组织中pERK蛋白表达水平。结果：免疫组织化学检测，pERK蛋白主要在海马齿状回（DG）颗粒细胞层和阿蒙角（CA）锥体细胞层神经元的细胞核中表达。E 18 d，海马DG和CA区pERK表达染色浅，排列稀疏，表达水平很低；E 18 d~P 7 d，pERK染色逐渐加深、密集，pERK表达水平逐渐升高，与其他6个时间点比较，P 7 d时pERK表达水平最高（F=34.537，P<0.01）。P 14~21 d，pERK染色逐渐变浅，密集程度逐渐降低，pERK表达水平逐渐下降（F=28.754，P<0.01）；P 28 d时pERK表达水平处于稳定的较低水平（F=6.075，P>0.05）。免疫印迹检测，各时间点均有pERK表达，E 18 d~P 7 d，pERK表达水平逐渐升高，与其他6个时间点比较，P 7 d时pERK表达水平最高（F=33.856，P<0.01）；P 14~21 d时pERK表达水平逐渐降低（F=22.627，P<0.01）；P 28 d时pERK表达处于稳定的较低水平（F=7.421，P>0.05）。结论：小鼠生后早期阶段，即E 18 d~P 7 d，海马发育明显增速，pERK表达水平也随之逐渐升高，P 7 d达高峰，随后逐渐下降直至稳定，pERK参与小鼠海马发育过程中的细胞增殖。

关键词: 胞外信号调节激酶, 海马发育, 昆明种小鼠, 免疫组织化学, 免疫印迹法

Abstract: Objective:To investigate the expressions of phosphorylated extracellular signal-regulated kinase (pERK) in the hippocampus of the mice of embryonic(E)18 d and postnatal(P)1,3,7,14,21 and 28 d,and to discuss its possible role during the hippocampus development of the mice. Methods:The hippocampus tissues of the mice of E 18 d,P 1 d,P 3 d,P 7 d,P 14 d,P 21 d and P 28 d were obtained.Each time point was used as observation group. Immunohistochemistry,Western blotting and image analysis technique were used to analyze the expression levels of pERK protein in hippocampus tissue at each time point. Results:The immunohistochemistry results revealed that pERK protein mainly expressed in the nucleus of the nerve cells in the granular layer of dentate gyrus and pyramidal layer of Ammon's horn in hippocampus. In the mice of E 18 d, the expression of pERK in DG and CA regions of hippocampus were slightly stained and arranged sparsely, and the expression level was low;and the pERK was deeply stained and the expression levels of pERK were increased gradually with the development of the hippocampus from E 18d to P 7 d;compared with other six time points, the expression level of pERK of the mice of P 7 d was the highest(F=34.537, P<0.01).From P 14 d to P 21 d,the pERK was slightly stained and the density was decreased;the expression levels of pERK were decreased (F=28.754,P<0.01),and then remained stably on P 28 d(F=6.075, P>0.05). The Western blotting results showed that pERK expressed at each time point,from E18 d to P7 d,the expression levels of pERK were increased gradually; compared with other six time points,the expression level of pERK on P 7 d was the highest (F=33.856, P<0.01).The expression levels of pERK were decreased from P 14 d to P 21 d(F=22.627,P<0.01),and then remained stably on P 28 d(F=7.421,P>0.05). Conclusion:The hippocampus development is increased significantly at the early stage of the mice(E18-P 7d),and the expression levels of pERK are increased gradually in the meanwhile;the expression level peaks on P 7 d, and then it is gradually decreased until a stable level. pERK may take part in the cell proliferation in the development of hippocampus of the mice.

Key words: extracellular signal-regulated kinase, hippocampus development,Kuming,mice, immunohistochemistry, immunoblotting method

中图分类号:

R329

王天月, 崔晓燕, 吴骁, 王丹. 磷酸化胞外信号调节激酶在小鼠海马发育过程中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 979-982.

WANG Tianyue, CUI Xiaoyan, WU Xiao, WANG Dan. Expression of phosphorylated extracellular signal-regulated kinase during development of hippocampus in mice[J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 979-982.

参考文献

[1] Chen H, Iinuma M, Onozuka M, et al. Chewing maintains hippocampus-dependent cognitive function[J].Int J Med Sci,2015,12(6):502-509.
[2] Serkova VV, Nikolskaya KA, Eremina LV. Hippocampus as an organizer of resent attention[J].Ross Fiziol Zh Im IM Sechenova,2015,101(6):678-688.
[3] Chowdhury I, Thompson WE, Thomas K. Prohibitins role in cellular survival through Ras-Raf-MEK-ERK pathway[J].Cell Physiol,2014,229(8):998-1004.
[4] Miller CR, Oliver KE, Farley JH. MEK1/2 inhibitors in the treatment of gynecologic malignancies[J].Gynecol Oncol,2014,133(1):128-137.
[5] Deschênes-Simard X, Kottakis F, Meloche S, et al. ERKs in cancer:friends or foes[J].Cancer Res,2014,74(2):412-419.
[6] Oliveros A, Cho CH, Cui A, et al.Adenosine A2A receptor and ERK-driven impulsivity potentiates hippocampal neuroblast proliferation[J].Transl Psychiatry, 2017,7(4):e1095.doi:10.1038/tp.2017.64
[7] Zhu YP, Xi SH, Li MY, et al.Fluoride and arsenic exposure affects spatial memory and activates the ERK/CREB signaling pathway in offspring rats[J].Neurotoxicology,2017,59:56-64.
[8] 孙艳秋,姜志梅,聂颖, 等.孤独症鼠模型脑组织CaMK Ⅱ和ERK表达的相关研究[J].中国儿童保健杂志,2017,25(7):679-682.
[9] Ha S,Radmond L.ERK mediates activity dependent neuronal complexity via sustained activity and CREB-mediated signaling[J].Dev Neurobiol,2018,68(14):1565-1579.
[10] Ohta KI, Suzuki S, Warita K, et al. Prolonged maternal separation attenuates BDNF-ERK signaling correlated with spine formation in the hippocampus during early brain development[J].J Neurochem,2017,141(2):179-194.
[11] 邵姝元,李潮,张敬坤,等.凋亡相关蛋白在小鼠海马发育和老化中的表达[J].解剖学杂志,2012,35(1):56-58.
[12] 席刚明, 汪华侨, 唐廷勇,等.胚胎时期大鼠海马结构的发育[J].四川解剖学杂志,1999,7(1):16-21.
[13] 卢瑛.抵抗素通过ERK1/2信号途径诱导人血管平滑肌细胞增殖[J].中国医药指南,2012,10(22):112-114.
[14] Chowdhury I, Thompson WE, Thomas K. Prohibitins role in cellular survival through Ras-Raf-MEK-ERK pathway[J].Cell Physiol,2014,229(8):998-1004.
[15] Formosa R, Vassallo J. cAMP signalling in the normal and tumorigenic pituitary gland[J].Mol Cell Endocrinol,2014,392(1/2):37-50.
[16] 佟雷,王振宇,季丽莉,等.ERK信号转导通路对新生大鼠海马神经干细胞增殖的影响[J].解剖科学进展,2011, 17(3):232-235.
[17] 刘俊杰,赵雅宁,陈禹廷,等.ERK信号通路抑制剂U0126对蛛网膜下腔出血大鼠早期脑损伤及神经元自噬的影响[J].西安交通大学学报:医学版, 2017, 38(1):18-23,28.
[18] 安朝旺,刘瑶,赵晓云,等.ERK1/2和PI3-K通路抑制剂对蛛网膜下腔出血大鼠海马区神经细胞自噬的协同作用[J].中国医科大学学报,2017,46(11):995-1000.
[19] 谭慧敏,郑兴荣,谭盛.ERK1/2信号通路对缺血后适应大鼠皮层的作用[J].中国实用神经疾病杂志,2017,20(15):1-5.
[20] Palmer TD, Markakis EA, Willhoite AR,et al.Fibroblast growth factor-2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS[J].J Neurosci,1999,19(19):8487-8497.
[21] Bayer SA.Development of the hippocampal region in the rat. Ⅰ. Neurogenesis examined with 3H-thymidine autoradiography[J].Comp Neurol,1980,190(1):87-114.

磷酸化胞外信号调节激酶在小鼠海马发育过程中的表达及其意义

Expression of phosphorylated extracellular signal-regulated kinase during development of hippocampus in mice

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李红霞, 宫海叶, 曾萧, 张素素, 刘伯锋. TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1041-1046.
[2]	赵振理, 畅继武, 孙光, 胡少华. AAX14401蛋白在人膀胱癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1052-1055.
[3]	张啸环, 段明华, 闫玉礼, 潘新, 李海清, 周长龙, 赵天倚. 人羊膜成纤维细胞的分离和鉴定[J]. 吉林大学学报(医学版), 2018, 44(04): 845-848.
[4]	阎慧, 娄石磊, 苗永迪, 岂蕊, 郭子硕, 邱悦, 孙聪. 大鼠结肠原代成纤维细胞的分离、培养和鉴定[J]. 吉林大学学报(医学版), 2018, 44(04): 849-852.
[5]	包幸福, 倪宇昕, 李莹, 郭小凯, 王玉琢, 方蛟, 胡敏. Wnt5a在小鼠牙骨质发育中的表达特点及其对成牙骨质细胞分化的影响[J]. 吉林大学学报(医学版), 2018, 44(03): 461-465.
[6]	马晓周, 刘麒麟, 尹唯凰, 王越, 于阳, 杨刚, 于子莹. CC趋化因子受体6在人唾液腺腺样囊性癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2018, 44(01): 147-150.
[7]	曹艳莎, 李婳, 陈明红, 刘宝琴, 王华芹, 李宁. 组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1177-1181.
[8]	高新雅, 张伟杰, 崔黎, 周学良, 马志俊, 吕转, 池艳艳, 王留兴. TM4SF1在乳腺癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1186-1192.
[9]	崔银星, 孔菲, 赵旭, 高普均. O-GlcNAc转移酶在肝细胞癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(05): 980-984.
[10]	周世平, 徐红, 王琇, 李蓥. 内镜黏膜下剥离术治疗食管颗粒细胞瘤2例报告并文献复习[J]. 吉林大学学报(医学版), 2017, 43(05): 1034-1036.
[11]	黄宝亮, 丁传波, 王佳奇, 郑毅男, 刘文丛, 张晶, 徐晓华. 红参中精氨酸双糖苷对小鼠的抗疲劳作用[J]. 吉林大学学报(医学版), 2017, 43(05): 897-902.
[12]	历春, 付天悦, 杨宝学, 盖晓东. 尿素通道蛋白B在人膀胱癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(04): 705-708.
[13]	石俊忠, 庄建彬, 张传山, 刘贤明, 孔大陆. β-tubulin Ⅲ在结肠腺癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(04): 770-775.
[14]	文爱平, 汪海娇, 金景姬, 蔡勇, 朱继红, 谌伦华. 子宫内膜癌组织中hMOF蛋白和mRNA表达及其临床意义[J]. 吉林大学学报(医学版), 2016, 42(06): 1138-1142.
[15]	温泉, 王园园, 司鹤南, 田亚萍. 成人郎格汉斯细胞组织细胞增生症1例报告及文献复习[J]. 吉林大学学报(医学版), 2016, 42(05): 985-987.