极低频电磁场暴露对SD大鼠肝、肾和脾脏功能及形态的影响

doi:10.13481/j.1671-587x.20180602

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (06): 1120-1123.doi: 10.13481/j.1671-587x.20180602

极低频电磁场暴露对SD大鼠肝、肾和脾脏功能及形态的影响

毛勇¹, 刘浩², 吴向阳¹, 高秉仁¹

1. 兰州大学第二医院心脏外科, 甘肃兰州 730000;
2. 河南省开封市中心医院心胸外科, 河南开封 475000

收稿日期:2018-03-15 出版日期:2018-11-28 发布日期:2018-11-28
通讯作者: 高秉仁,教授,博士研究生导师(Tel:0931-5190572,E-mail:gaobingren2016@163.com) E-mail:gaobingren2016@163.com
作者简介:毛勇(1989-),男,甘肃省天水市人,住院医师,医学硕士,主要从事瓣膜性心脏病及人工血泵方面的研究。
基金资助:
国家自然科学基金资助课题（81470026）；中央高校科研项目资助课题（lzujbky-2015-47）

Effects of extremely low frequency electromagnetic fields exposure on function and morphology of liver,kidney and spleen of SD rats

MAO Yong¹, LIU Hao², WU Xiangyang¹, GAO Bingren¹

1. Department of Cardiac Surgery, Second Hospital, Lanzhou University, Lanzhou 730000, China;
2. Department of Cardiothoracic Surgery, Central Hospital, Kaifeng City, Henan Province, Kaifeng 475000, China

Received:2018-03-15 Online:2018-11-28 Published:2018-11-28

摘要/Abstract

摘要： 目的：探讨极低频电磁场（ELF-EMF）暴露对SD大鼠肝、肾和脾等脏器功能及形态的影响，阐明ELF-EMF辐射损伤的机制。方法：20只雄性SD大鼠随机分为暴露组和对照组，每组10只。暴露组大鼠暴露于磁感应强度为0.1 T、频率为50 Hz的ELF-EMF装置中，每天8 h，连续暴露30 d；对照组大鼠不进行上述电磁场暴露。暴露30 d后，检测2组大鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、尿素氮（UN）和肌酐（Cr）水平，HE染色检测2组大鼠肝脏、肾脏和脾脏形态表现。结果：与对照组比较，暴露组大鼠血清ALT、AST、UN和Cr水平明显升高（P<0.05）。暴露组大鼠肝脏中央静脉及肝血窦、肾脏肾小球及间质毛细血管以及脾脏脾血窦均出现扩张充血，且部分肝细胞出现坏死；对照组大鼠肝脏、肾脏和脾脏形态无异常。结论：ELF-EMF （0.1 T、50 Hz）暴露影响SD大鼠肝、肾和脾脏功能及结构，其机制可能与ELF-EMF暴露引起的氧化应激反应有关联。

关键词: 极低频电磁场, 大鼠,SD, 肝脏, 肾脏, 脾脏

Abstract: Objective: To discuss the effects of extremely low frequency electromagnetic fields(ELF-EMF) exposure on the function and morphology of liver,kidney and spleen of the SD rats, and to clarify the mechanism of radiation damage of ELF-EMF.Methods: Twenty male SD rats were randomly divided into exposure group and control group (n=10). The rats in exposure group were exposed in an ELF-EMF device with a magnetic induction of 0.1T and a frequency of 50 Hz; the exposure was conducted for 8 h per day for 30 d. The rats in control group didn't receive any treatment. After exposure for 30 d, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (UN), creatinine (Cr) of the rats in two groups were detected. HE staining was used to detect the morphology of the liver, kidney, spleen tissues of the rats in two groups.Results: Compared with control group, the serum levels of ALT, AST, UN, and Cr of the rats in exposure group were increased(P<0.05). In exposure group,the dilatation and congestion were found in the central veios of liver and hepatic sinusoids, glomeruli and interstitial capillaries of kidney, and insinusoids of spleen of the rats; part of the hepatocytes showed necrosis. There was no abnormality morphology in the liver, kidney,and spleen tissues of the rats in control group.Conclusion: Exposure to ELF-EMF(0.1 T, 50 Hz) can affect the function and structures of liver, kidney and spleen tissues of the SD rats, and its mechanism may be related to oxidative stress induced by ELF-EMF.

Key words: extremely low frequency electromagnetic fields, rats,SD, liver, kidney, spleen

中图分类号:

R144.1

毛勇, 刘浩, 吴向阳, 高秉仁. 极低频电磁场暴露对SD大鼠肝、肾和脾脏功能及形态的影响[J]. 吉林大学学报(医学版), 2018, 44(06): 1120-1123.

MAO Yong, LIU Hao, WU Xiangyang, GAO Bingren. Effects of extremely low frequency electromagnetic fields exposure on function and morphology of liver,kidney and spleen of SD rats[J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1120-1123.

参考文献

[1] de Bruyn L, de Jager L. Effect of long-term exposure to a randomly varied 50 Hz power frequency magnetic field on the fertility of the mouse[J].Electromagn Biol Med,2010,29(1/2):52-61.
[2] 邵建华,叶红.极低频电磁场的生物效应及其医学应用[J].上海中医药大学学报,2010,24(4):94-98.
[3] Johansson O. Disturbance of the immune system by electromagneticfields-A potentially underlying cause for cellular damage and tissue repairreduction which could lead to disease and impairment[J]. Pathophysiology, 2009,16(2/3):157-177.
[4] 王少霞.极低频电磁场暴露对大鼠心脏组织结构的影响[A].第九届全国生物医学体视学学术会议、第十二届全军军事病理学学术会议暨第八届全军定量病理学学术会议论文集[C].呼和浩特,2014:261-262.
[5] 张安英,周玉荣,邓波,等.红外光谱法研究极低频电磁场暴露对大鼠肺组织二级结构的影响[J].中国生物医学工程学报,2009,28(1):65-70.
[6] Yokus B, Cakir DU, Akdag MZ, et al. Oxidative DNA damage in rats exposed to extremely low frequency electromagnetic fields[J]. Free Radic Res, 2005, 39(3):317-323.
[7] Rosen AD. Mechanism of action of moderate-intensity static magnetic fields on biological systems[J]. Cell Biochem Biophys,2003,39(2):163-173.
[8] Carpenter DO. Human disease resulting from exposure to electro magnetic fields[J]. Rev Environ Health,2013,28(4):159-172.
[9] 文晓成. 极低频磁场影响血液流态的初步研究[D].西安:第四军医大学,2012.
[10] 孙兆钰,耿读艳,陈传芳.极低频磁场对小鼠胚胎成纤维细胞中隐花色素生物节律的影响[J]. 中华劳动卫生职业病杂志,2017,35(6):459-462.
[11] Kuzay D,Ozer C,Sirav B,et al. Oxidative effects of extremely low frequency magnetic field and radio frequency radiation on testes tissues of diabetic and healthy rats[J]. Bratisl Lek Listy,2017,118(5):278-282.
[12] Ravera S,Repaei E,Morelli A,et al.Effects of extremely low frequency electromagnetic fields on the adenylate kinase activity of rod outer segmentof bovine retina[J].Bioelectromagnetics,2004,25(7):545-551.
[13] Destefanis M,Viano M,Leo C,et al. Extremely low frequency electromagnetic fields affect proliferation and mitochondrial activity of human cancer cell lines[J].Int J Radiat Biol,2015,91(12):964-972.
[14] 耿读艳,徐桂芝,万晓伟,等. 模拟输电环境电磁场对动物血清和细胞超微结构的影响[J]. 高电压技术,2013,39(1):163-174.
[15] Hashish AH, EL-MissiryMA, Abdelkader HI, et al. Assessment of biological changes of continuous whole body exposure to static magnetic field and extremely low frequency electromagnetic fields in mice[J]. Ecotoxicol Environ Safety,2008,71(3):895-902.
[16] Pi J,Zhang Q,Fu J,et al. ROS signaling,oxidative stress and Nrf2 in pancreatic beta-cell function[J]. Toxicol Appl Pharmacol,2010,244(1):77-83.
[17] 郝帅,张颖,周良,等. 极低频电磁场对小鼠血清趋化因子水平的影响[J].环境与健康杂志,2017,34(5):382-385.
[18] 孙跃,兰天,郭姣.鞘氨醇激酶信号通路在肝纤维化中的作用机制[J].临床肝胆病杂志,2017,33(9):1798-1801.

极低频电磁场暴露对SD大鼠肝、肾和脾脏功能及形态的影响

Effects of extremely low frequency electromagnetic fields exposure on function and morphology of liver,kidney and spleen of SD rats

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