基础转录激活因子1蛋白在胃癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20190118

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (01): 94-99.doi: 10.13481/j.1671-587x.20190118

• 临床研究 • 上一篇

基础转录激活因子1蛋白在胃癌组织中的表达及其临床意义

穆天驰¹, 刘超², 富帅¹, 李启阳¹, 王馨梦¹, 肖建英¹

1. 锦州医科大学基础医学院生物化学与分子生物学教研室, 辽宁锦州 121001;
2. 锦州医科大学基础医学院发育生物学教研室, 辽宁锦州 121001

收稿日期:2018-01-26 发布日期:2019-01-28
通讯作者: 肖建英,教授,硕士研究生导师(Tel:0416-4673936,E-mail:xiaojianying@lnmu.edu.cn) E-mail:xiaojianying@lnmu.edu.cn
作者简介:穆天驰(1989-),男,辽宁省锦州市人,在读医学硕士,主要从事分子生殖和分子肿瘤学方面的研究。

Expression of activator of basal transcription 1 in gastric cancer tissue and its clinical significance

MU Tianchi¹, LIU Chao², FU Shuai¹, LI Qiyang¹, WANG Xinmeng¹, XIAO Jianying¹

1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121001, China;
2. Department of Developmental Biology, School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121001, China

Received:2018-01-26 Published:2019-01-28
Contact: 国家自然科学基金资助课题（81270698，31371173，81401199）；辽宁省科技厅科技计划项目资助课题（2015020697） E-mail:xiaojianying@lnmu.edu.cn

摘要/Abstract

摘要： 目的：探讨基础转录激活因子1（ABT1）蛋白在胃癌组织中的表达及其与胃癌患者临床参数和预后的相关性，阐明ABT1在胃癌发生发展中的作用。方法：选择100例胃癌患者的胃癌组织和80例与其配对的癌旁组织，采用免疫组织化学法检测ABT1蛋白在癌组织及癌旁组织中的表达情况。对免疫组织化学结果中染色细胞的比例和染色程度进行半定量分析，对半定量分析结果与胃癌患者临床参数的关系进行统计学分析。采用Kaplan-Meier法分析ABT1蛋白表达水平与胃癌患者生存期的相关性。结果：ABT1蛋白在胃癌组织和癌旁组织中的细胞核和细胞质中均有表达，且胃癌组织中ABT1蛋白表达水平低于癌旁组织（P=0.021）；胃癌患者胃癌组织中ABT1蛋白表达水平与胃癌组织病理分级呈负相关关系（r=-0.224，P=0.026）。Kaplan-Meier生存曲线分析，胃癌患者ABT1蛋白高表达与预后良好呈正相关关系（HR=1.483，P<0.01），且ABT1蛋白高表达的胃癌患者生存率明显高于ABT1蛋白低表达的胃癌患者（HR=2.411，P=0.027）。结论：ABT1蛋白在胃癌组织中低表达，提示其可作为胃癌预后良好的标志因子之一。

关键词: 基础转录激活因子1, 胃肿瘤, 免疫组织化学, 组织芯片

Abstract: Objective: To investigate the expression of activator of basal transcription 1 (ABT1) protein in gastric cancer tissue and its relationships with the clinical parameters and prognosis of gastric cancer patients, and to clarify the role of ABT1 in the occurrence and development of gastric cancer.Methods: A total of 100 cases of cancer tissue of the gastric cancer patients and 80 pairs of adjacent tissue were selected. The expressions of ABT1 in cancer tissue and adjacent tissue were detected by immunohistochemistry and the proportion of stained cells and the degree of staining in the immunohistochemistry results were analyzed using semi-quantitative analysis. The relationships between the semi-quantitative analysis results and the clinical parameters of gastric cancer patients were statistically analyzed. Kaplan-Meier method was used to analyze the correlation between the ABT1 protein expression level and the survival of gastric cancer patients.Results: ABT1-positive staining was observed in the nucleus and cytoplasm of gastric cancer tissue and adjacent gastric tissue. The expression level of ABT1 in gastric cancer tissue was lower than that in adjacent tissue(P=0.021). The ABT1 protein expression level in gastric cancer tissue was significantly negatively correlated with the pathological grade (r=-0.224,P=0.026).The Kaplan-Meier analysis results of the survival curve showed that the high expression of ABT1 was associated with good prognosis in the gastric cancer patients (HR=1.483,P<0.01).The survival rate of gastric cancer patients with high ABT1 expression was significantly higher than that of the patients with low ABT1 expression (HR=2.411,P=0.0272).Conclusion: The expression of ABT1 in gastric cancer tissue is lower,indicating that ABT1 can be used one of the markers of good prognosis of gastric cancer.

Key words: ABT1, stomach neoplasms, immunohistochemistry, tissue microarray

中图分类号:

R735.2

穆天驰, 刘超, 富帅, 李启阳, 王馨梦, 肖建英. 基础转录激活因子1蛋白在胃癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(01): 94-99.

MU Tianchi, LIU Chao, FU Shuai, LI Qiyang, WANG Xinmeng, XIAO Jianying. Expression of activator of basal transcription 1 in gastric cancer tissue and its clinical significance[J]. Journal of Jilin University(Medicine Edition), 2019, 45(01): 94-99.

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基础转录激活因子1蛋白在胃癌组织中的表达及其临床意义

Expression of activator of basal transcription 1 in gastric cancer tissue and its clinical significance

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