关键词: 肿瘤细胞, 培养的, 吗啡, 转录因子, TFⅡ类, 基因表达, 逆转录聚合酶链反应

Abstract: Objective To determine the effect of morphine on RNA synthesis of C6 glioma cells. Methods Gene expression level of TFⅡF was detected by RT-PCR. Results Exposure of morphine to C6 glioma cells caused a decrease of TFⅡF gene expression as compared with corresponding control cells, and the effect could be reversed by naloxone. Conclusion The down-regulation of morphine on gene expression of TFⅡF may be mediated by μreceptor.

Key words: umor cells, cultured, morphine, transcription factors, TFⅡ, gene expression, reverse transcriptase polymerase chain reaction