关键词: 肝细胞, 基因, 转染, 共刺激分子

Abstract: Objective To study the killing effects of induced LAK-cells on B₇ gene transfected cells of liver carcinoma. Methods A new vector pRCCMV-B_7-1 containing B_7-1 gene was constructed to establish the new cell line B₇⁺SMMC7721 through transfection B₇ gene into the hepatocarcinoma cell SMMC7721 by liposome-mediated transduction. RT-PCR and flow cytometry were used to identify the expression of the transfected gene. MTT assay was used to evaluate the killing effects of LAK cells activated by IL-2 on the transfected line and the wild type of the cells.　Results B₇ gene was steadily expressed in B₇⁺SMMC7721 and LAK cells had effectively killing effects on the B₇⁺SMMC7721 cells. Conclusion B₇ gene can be transfected into hepatocarcinoma cells and can be expressed steadily in vitro, which can increase the efficiency of LAK cells activated by IL-2 on them.

Key words: hepatocellular, gene, transfection, costimulatory molecule