关键词: 实验性, 疾病模型, 动物, 四氯化碳, 乙醇

Abstract: Objective To study the establishment and stability of hepatic cirrhosis animal model(CM). Methods The methods of compound factor, carbon tetrachloride(CCl₄) plus alcohol, corn flour and cholesterol plus alcohol were used to construct the hepatic cirrhosis rat models. 80 Wistar rats were selected, male and female were ,respectively. There were 40 Wistar rats treated with compound factor method.The fodder feed was 80% corn flour,20% animal oil, and 0.5% cholesterol, the only beverage was 5% alcohol .At the first time,the Wistar rats were administered with 40% CCl₄-olive oil mixture by subcutaneous injection (5 mL·kg_-1). Then they were hypodermically injected with 40% CCl₄-olive oil mixture (3 mL·kg^-1) every 3 days for six weeks. At the end of the 6th week,six Wistar rats were executed randomly and made into hepatic smears which were accorded with hepatic cirrhosis (The forming of pseudolobuli was the only standard to decide the successful establishment of CM). The rest rats were randomly divided into two groups. There were 13 Wistar rats treated with normal fodder feeding in each group. Meanwhile the rats in one group were hypodermically injected with 40% CCl₄-olive oil mixture (3 mL·kg^-1) every 7 days. Then the pathological changes at the end of 7th and 8th week were observed. Twenty Wistar rats treated with CCl₄ plus alcohol were fed with normal fodder food. Remain ing conditions were as same as those were used in the first 6 weeks of compound factor method. Twenty Wistar rats treated with corn flour and cholesterol plus alcohol weren′t hypodermically injected with CCl₄ and remaining conditions were same with those were used in the first 6 weeks of compound factor method.Results The rat models with hepatic cirrhosis treated with compound factor were established completely at the end of 6th week. Then the rats were fed with normal fodder food. Pseudolobuli were all disappeared after two weeks. And the rats were hypodermically injected with CCl₄ for 2 weeks after establishment of CM,pseudolobuli were mainly existed. Typical CM were not constructed with other two methods. Conclusion The forming rate of hepatic cirrhosis model constructed with compound factor is high. After establishment of the model, CCl₄ can keep the relative stability of the models.

Key words: experimental, disease model, animal, carbon tetrachloride, alcohol