关键词: 酶学, 新生血管化, 病理性, 一氧化氮合酶, 血管生成因子, 免疫组织化学

Abstract: Objective To study the expression of inducible nitric oxide synthase(iNOS) in lung carcinoma and its relation with clinical parameters and tumor angiogenesis. Methods The expressions of iNOS mRNA, protein and tumor interstitial MVD were detected in 41 lung carcinoma tissues by the method of in situ hybridization and immunohistochemistry. Results The expressions of iNOS mRNA and protein in lung carcinoma was significantly higher than that of peri-carcinoma and normal tissues(P<0.005). The positive rates were 60.98% and 58.54%, respectively. The positive staining was mostly localized in cytoplasm of tumor cells and some macrophages and neutrophils. There were positive correlations between iNOS expression and clinical stages and lymph node metastasis, and the MVD of iNOS positive group was obviously higher than that of negative group significantly. Conclusion The iNOS expression in lung carcinoma may be valuable in assessing lymph node metastasis and clinical stages for patients before operation. As the iNOS is able to promote angiogenesis, the inhibition of iNOS may become a new target for anti-angiogenic therapy.

Key words: enzymology, neovascularization, pathologic, nitro-oxide synthase, angiogenesis factor, immunohistochemistry