盆底肌损伤后修复过程中囊泡转运相关基因的表达

doi:10.13481/j.1671-587X.20210503

Abstract

Abstract: Objective

To explore the gene expressions related to skeletal muscle regeneration and repair and vesicle transport in the process of pelvic floor muscle injury caused by vaginal delivery， and to preliminarily clarify the potential role of effector molecules such as ADP ribosylation factor GTPase activated protein 3（ARFGAP3） in the regeneration and repair of pelvic floor muscles after injury.

Methods

Thirty-two female healthy 12-week-old C57 BL/6 mice were selected， and the pelvic floor muscle injury models were constructed by vaginal expansion and distal traction. They were divided into control group， modeling 1 d group， and modeling 3 d group and modeling 5 d group. In the Gene Expression Omnibus （GEO）， “muscle regeneration” was used as a keyword to search for information related to skeletal muscle regeneration and repair， three data sets GSE5413， GSE45577 and GSE469 were selected， and GEO2R online analysis tool and R software were used to screen for the differential expression genes； among the co-differentially expressed genes， 10 genes related to intracellular vesicle transport were selected （ArfGAP3， LGALS3， KDELR3， CSF1R， ANXA4， STMN1， THBS2， PLXNB2， KIF20A， EMR1）. Real-time fluorescence quantitative PCR（RT-qPCR） method was used to detect the expression levels of the above-mentioned vesicle transport-related differential genes in pelvic floor muscle of the mice during the repair of pelvic floor muscle injury.

Results

The genes whose expression levels were increased after pelvic floor muscle injury were ARFGAP3， STMN1， THBS2 and PLXNB2. Compared with control group， the expression levels of ARFGAP3， STMN1 and THBS2 in the pelvic floor muscle tissue of the mice in modeling 1 d group were increased after injury（P<0.05 or P<0.01）， and the expression levels of ARFGAP3 and PLXNB2 in modeling 3 d group were increased after injury （P<0.01）. CSF1R， ANXA4 and EMR1 were the genes whose expression levels were reduced after pelvic floor muscle injury. Compared with control group， the expression levels of CSF1R and EMR1 in modeling 1 d group were decreased after injury（P<0.05 or P<0.01）； the expression levels of CSF1R and ANAX4 in modeling 3 d group after injury were decreased（P<0.05）；the expression level of ANAX4 in modeling 5 d group was decreased（P<0.05）. The expression levels of LGARLS3， KDELR3 and KIF20A remained unchanged after pelvic floor muscle injury.

Conclusion

ARFGAP3 may play an important regulatory role in the repair process of pelvic floor muscles after injury.

Key words: pelvic floor muscle injury, muscle regeneration, vesicle transport, ADP ribosylation factor GTPase activated protein 3, gene expression

CLC Number:

R711.4

Jue CHEN,Li HONG,Suting LI,Zhi WANG,Mao CHEN,Menglei HAO,Ya XIAO,Jie MIN,Ming HU. Expressions of vesicle transport-related genes during repair of pelvic floor muscles after injury[J].Journal of Jilin University(Medicine Edition), 2021, 47(5): 1092-1098.

Figures/Tables 4

Tab.1

Fig.1

Fig. 2

Fig. 3

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Gene	Forward	Reverse
ARFGAP3	5'-CGAGGAGGAAGTACCAGGAGGAC-3'	5'-CCAGTAGGAATCGGCACCATCATC-3'
LGARLS3	5'-CAGGACAGGCTCCTCCTAGTGC-3'	5'-GCAGCAGGATAGCCTCCAGGAG-3'
KDELR3	5'-TGAACTACAGTTACACGCCGATGG-3'	5'-AGTGGTGATGGTCTCAGCCTCTC-3'
CSF1R	5'-CCTCCTCTGGTCCTGCTGCTG-3'	5'-TTCCACACTGCCATTGCTCACAC-3'
ANXA4	5'-GGCTTAGGCACTGATGACAACACC-3'	5'-CACAGAGAACGAGCAGAACCTTCC-3'
STMN1	5'-GCAAGATGGCGGAGGAGAAGC-3'	5'-TTGTTCTTCCGCACCTCTTCCAC-3'
THBS2	5'-CAGATTGGCTACGCAGGTGATGG-3'	5'-CATCGTCCTCGTCACAAGCATCTC-3'
PLXNB2	5'-CAGAAGCGAGACACGGCACAC-3'	5'-TACCATGACCAGAGGCAGGATGAG-3'
KIF20A	5'-CCAGCAATCCAGCCGCAGTC-3'	5'-TCAGAGCCAGCCAGGTCACAG-3'
EMR1	5'-TGGCAAGCATCATGGCATACCTG-3'	5'-TGACGGTTGAGCAGACAGTGAATG-3'
GAPDH	5'-AGGAGCGAGACCCCACTAACA-3'	5'-AGGGGGGCTAAGCAGTTGGT-3'

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Expressions of vesicle transport-related genes during repair of pelvic floor muscles after injury

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