The cell membrane of HEK-293T cells was separated by ultracentrifugation，and the NVs were prepared by liposome extrusion instrument； the particle size of NVs was detected by dynamic light scattering， and the ultrastructure of NVs was observed under transmission electron microscope. The surival rates of dendritic DC2.4 cells of the mice after treated with different concentrations （5， 10， 20， 50， 75 and 100 mg·L^－1）of NVs were detected. The endocytosis of the vesicles was analyzed under laser confocal fluorescence microscope. The OXA was loaded into the lumen of the vesicles by electrioporation or incubation to obtain the nano drugs NVs@OXA.The loading efficiency and changes of particle sizes of the nano drugs NVs@OXA were detected.The free OXA was selected as control（free OXA group），the survival rates of colon cancer CT26 cells treated with different concentrations （1.0， 2.5， 5.0， 10.0 and 15.0 μmol·L^－1） of NVs@OXA （NVs@OXA groups）were detected by MTT method， and the apoptotic rates of the colon cancer CT26 cells in various groups was detected by flow cytometry.

The NVs with an average particle size of 222.2 nm were prepared by cell membrane.The cytocompatibility test results showed that the survival rates of all the dendritic DC2.4 cells of the mice treated with NVs were >100%； the loading efficiency of OXA prepared by electrioporation method was higher than that prepared by incubation method. There was no significant change in the particle size of OXA during 12 d after the preparation of nano drugs. Under laser confocal fluorescence microscope， the NVs@OXA could successfully enter the colon cancer CT26 cells. The MTT detection results showed that the survival rates of the colon cancer cells in NVs@OXA groups were lower than that in free OXA group when the concentrations of OXA were 10 and 15 μ mol·L^－1 （P<0.05）.The flow cytometry results showed that the apoptotic rates of the colon cancer cells in NVs@OXA group was higher than that in free NVs group （P<0.05）.

The OXA-loaded nanomedicine NVs@OXA is successfully prepared by NVs.The electroporation method has a higher OXA-loading efficiency than the incubation method. NVs@OXA can be internalized into the CT26 cells. NVs@OXA shows a stronger tumor killing effect than the free OXA.