A lentiviral vector expressing Pin1 shRNA was constructed and used to infect the cells， and shCaski cells were obtained. The Caski， shCaski and C33A cells in the logarithmic growth phase were selected and divided into normal control group and 10， 20， 50 and 100 μmol·L^－1 juglone groups. After juglone treatment for 24 h， the cell proliferation activities in various groups were detected by MTT method. The Caski cells and C33A cells in the logarithmic growth phase were selected and divided into control group and 20 μmol·L^－1 juglone group. After 24 h of treatment of the cells in each group， the percentages of cells in different cell cycles and the early apoptotic rates in various groups were detected by flow cytometry. Hoechst33258 fluorescence staining was used to observe the morphology of nucleus； Western blotting method was used to detect the expressions of cell cycle and apoptosis-related proteins.

The MTT experiment showed that the proliferation activities of the Caski cells in different concentrations of juglone groups were significantly decreased （P<0.05or P<0.01） compared with normal control group， while the proliferation activities of C33A and shCaski cells in 50 and 100 μmol·L^－1 juglone groups were significantly decreased（P<0.05）.The results of Hoechst 33258 staining showed that compared with control group， both the Caski cells and C33A cells in 20 μmol·L^－1juglone group showed increased nuclear fragmentation， and the number of nuclei fragmentation of Caski cells was more than that of C33A cells. The results of flow cytometry showed that compared with control group， the percentage of Caski cells in G₂ phase in 20μmol·L^－1 juglone group was increased significantly （P<0.01）； while there was no significant difference in the percentage of C33A cells in G₂ phase in 20 μmol·L^－1 juglone group（P>0.05）；compared with control group，the early apoptotic rate of Caski cells in 20 μmol·L^－1 juglone group was increased significantly （P<0.01）； while the early apoptotic rate of C33A cells in 20 μmol·L^－1 juglone group was also increased， but there was no significantly difference（P>0.05）. The Western blotting results showed that compared with the C33A cells，the expression amount of Pin1 protein in the Caski cells was more higher； and the expression amount of Pin1 protein in Caski and shCaski cells in 20 μmol·L^－1 juglone group were decreased. Compared with control group， the expression amounts of cell cycle-related proteins in the C33A cells in 20 μmol·L^－1 juglone group did not change significantly； the expression amount of pATM， pChk2， pCdc25c Ser216 and pCdc25c Tyr216 in the Caski cells in 20 μmol·L^－1 juglone group were increased， while the expression amount of pCdc25c protein was decreased in 20 μmol·L^－1 juglone group；the expression amount of Cdk1 protein didn’t change obviously. Compared with control group， the amounts of Bcl-2 and mitochondrial CytC Caski cells in 20 μmol·L^－1 juglone group were decreased， but the expression amounts of cytoplasmic CytC，Bax，Cleaved Caspase-3 and Cleaved PARP proteins in the Caski cells in 20 μmol·L^－1 juglone group were increased.

Juglone has stronger inhibitory and pro-apoptotic effects in the HPV-positive cervical cancer cells than negative cells； its mechanism may be related to the specific inhibition of Pin1 gene by juglone.