Journal of Jilin University(Medicine Edition) ›› 2023, Vol. 49 ›› Issue (1): 55-66.doi: 10.13481/j.1671-587X.20230108
• Research in basic medicine • Previous Articles Next Articles
Xiaohui WU1,Fan YANG1,Xinru GUO1,Ke YUAN1,Zongyi MA1,Ting LIAN1,Rajiv Kumar JHA2()
Received:
2022-03-11
Online:
2023-01-28
Published:
2023-02-03
Contact:
Rajiv Kumar JHA
E-mail:rajiv12@hotmail.com
CLC Number:
Xiaohui WU,Fan YANG,Xinru GUO,Ke YUAN,Zongyi MA,Ting LIAN,Rajiv Kumar JHA. Effect of Orlistat combined with EZH2 inhibitor GSK126 on lipid metabolism reprogramming in pancreatic cancer cells[J].Journal of Jilin University(Medicine Edition), 2023, 49(1): 55-66.
Tab.1
Primer sequences of RT-qPCR"
Gene | Forward (5'—3') | Reverse (5'—3') |
---|---|---|
Caspase-1 | GCCTGTTCCTGTGATGTGGAG | TGCCCACAGACATTCATACAGTTTC |
Caspase-3 | GACTCTGGAATATCCCTGGACAACA | AGGTTTGCTGCATCGACATCTG |
Caspase-7 | ATGTCACCATGCGATCCATCA | TCCGTTTCGAACGCCCATA |
Caspase-9 | CTTGAGACACTCGCTCAGCTTC | CTTGAGACACTCGCTCAGCTTC |
CD133 | AGTGGCATCGTGCAAACCTG | CTCCGAATCCATTCGACGATA |
CD24 | CTCCTACCCACGCAGATTTATT | GTGGTGGCATTAGTTGGATTTG |
CD44 | GAAATGGCACCACTGCTTATG | CTACTAGGAGTTGCCTGGATTG |
VEGFA | AGGGCAGAATCATCACGAAGT | AGGG TCTCGATTGGATGGCA |
E-cadherin | ATCAAAGGTATCACGGCAAACG | CGGAGAGCTCGTCCACGTAT |
FASN | CTAGGTTTGATGCCTCCTTCTT | GATGGCTTCATAGGTGACTTCC |
ACACA | CTTGCTAGGGCAGAAGGTATTC | CCCAGGCCACATGAAACATA |
CS | GAGCAGGGTAAAGCCAAGAA | CCAAACAGGACCGTGTAGTAAT |
ACLY | GTCTCATCGGAGTCGCATTT | CTCCTTCCCAGCACAAAGAT |
SCD | GCACATCAACTTCACCACATTC | GTAGTTTCCATCTCCGGTTCTT |
ACSS2 | ACGCTTTGAGACAACCTACTT | CCTGCCAGTGATCCAGTAATAG |
β-actin | ATTGCCGACAGGATGCAGA | GAGTACTTGCGC TCAGGAGGA |
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