MiR-491-5p过表达对人鼻咽癌HONE-1细胞增殖和迁移的影响

doi:10.13481/j.1671-587X.20230504

Abstract

Abstract:

Objective To discuss the effect of microRNA-491-5p（miR-491-5p） over-expression on the proliferation and migration of the human nasopharyngeal carcinoma HONE-1 cells， and to provide the evidence for the study of the pathogenesis and targeted therapy of nasopharyngeal carcinoma. Methods The human nasopharyngeal carcinoma HONE-1 cells were divided into control group （transfected with control plasmid） and miR-491-5p group （transfected with miR-491-5p plasmid）. The transfection efficiencies of the HONE-1 cells in two groups were observed under fluorescence microscope； the proliferation activities of the HONE-1 cells in two groups were detected by CCK-8 assay； the number of migration HONE-1 cells in two groups were detected by Transwell chamber assay；the expression levels of Vimentin and E-cadherin mRNA in the HONE-1 cells in two groups were detected by real-time fluorescence quantitative PCR（RT-qPCR）method； the expression levels of Vimentin and E-cadherin proteins in the HONE-1 cells in two groups were detected by Western blotting method. Results Compared with control group， the proliferation activities（t=2.832， P=0.0473； t=4.522， P=0.0014； t=9.308， P<0.01） and number of migration HONE-1 cells （t=9.639， P<0.01） in miR-491-5p group at 24，48，and 72 h after treatment were significantly decreased； compared with control group， the expression levels of Vimentin mRNA （t=7.535， P=0.0017） and protein （t=7.219， P=0.0187） in the HONE-1 cells in miR-491-5p group were significantly decreased， and the expression levels of E-cadherin mRNA （t=4.88， P=0.0395） and protein （t=5.754， P=0.0289） were significantly increased. Conclusion Over-expression of miR-491-5p can inhibit the proliferation， migration， and epithelial-mesenchymal transition （EMT） of the human nasopharyngeal carcinoma cells.

Key words: MicroRNA-491-5p, Nasopharyngeal carcinoma, Cell proliferation, Cell migration, Epithelial-mesenchymal transition

CLC Number:

R739.62

Dandan WANG,Ning ZHOU,Dongqin LIU,Jie ZHAO,Chao LIANG,Juanjuan DAI,Yan WU. Effect of miR-491-5p over-expression on proliferation and migration of human nasopharyngeal carcinoma HONE-1 cells[J].Journal of Jilin University(Medicine Edition), 2023, 49(5): 1134-1139.

Figures/Tables 5

References 23

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Effect of miR-491-5p over-expression on proliferation and migration of human nasopharyngeal carcinoma HONE-1 cells

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