前列腺素E2对雌性小鼠下丘脑视前区正中核热敏神经元放电活动的影响及其机制

doi:10.13481/j.1671-587X.20250103

Abstract

Abstract:

Objective To discuss the effect of prostaglandin E2 （PGE₂）， a pyrogenic mediator， on the discharge activity of warm-sensitive neurons （WSNs） in median preoptic nucleus （MnPO） of hypothalamus in the female mice， and to clarify its mechanism. Methods Coronal brain slices of MnPO were prepared from the female mice. The slices were then perfused with artificial cerebrospinal fluid （ACSF） containing synaptic transmission blockers （STBs）. The discharge frequency was monitored using the patch clamp technique while changing the temperature of the perfusate to identify the WSNs. A total of 32 MnPO WSNs were divided into base line group（n=32） and PEG₂ （n=32）. The patch clamp technique was employed to monitor the discharge frequencies of MnPO WSNs following the perfusion of ACSF and 1 μmol·L^-1 PGE₂， respectively. The MnPO WSNs with good activity and significant change in discharge frequency after PGE₂ perfusion were divided into PGE₂ receptor E-series prostaglandin receptrol （EP）1 antagonist （EP1 ant）+PGE₂ group （n=7）， EP3 ant+PGE₂ group （n=7）， and EP4 ant+PGE₂ group （n=7）. The patch clamp technique was used to monitor the discharge frequencies of MnPO WSNs following the perfusion of 3 μmol·L^-1 EP1 ant and 1 μmol·L^-1 PGE₂ mixture， 10 μmol·L^-1 EP3 ant and 1 μmol·L^-1 PGE₂mixture， and 10 μmol·L^-1 EP4 ant and 1 μmol·L^-1 PGE₂ mixture， respectively. Resluts： After perfuson of the ACSF containing STBs， a total of 188 MnPO neurons from the female mice with an intrinsic temperature sensitivity coefficient （m value） were identified； out of these， 32 neurons had an m value of ≥0.8 and were identified as MnPO WSNs， accounting for approximately 17% of all recorded neurons. Compared with baseline discharge frequency， the discharge frequency of MnPO WSNs after addition of PGE2 was decreased （P<0.05）. Compared with PGE₂ group， the percentage change in discharge frequency of MnPO WSNs in EP3 ant+PGE₂ group was significantly decreased （P<0.05）. Compared with PGE₂ group， the percentage change in discharge frequency of MnPO WSNs in EP1 ant+PGE₂ group had no significant difference （P>0.05）. Compared with PGE₂ group， the percentage change in discharge frequency of MnPO WSNs in EP4 ant+PGE₂ group had no significant difference（P>0.05）. Conclusion In the female mice， WSNs make up approximately 17% of the total neurons in MnPO. PGE₂ can directly inhibit the discharge activity of MnPO WSNs in the female mice through postsynaptic mechanism involving EP3 receptors.

Key words: Fever, Prostaglandin E2, Median preoptic nucleus, Warm-sensitive neuron, EP3 receptor

CLC Number:

R714

Xiaoyu HOU,Ya LI,Yian SONG,Tianhui HE,Jie ZHANG,Jianhui XU. Effect of prostaglandin E2 on discharge activity of warm-sensitive neurons in median preoptic nucleus of hypothalamus in female mice and its mechanism[J].Journal of Jilin University(Medicine Edition), 2025, 51(1): 17-25.

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References 25

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Group	Discharge frequency（Hz）		Percentage change in discharge frequency （η/%）
Group	Baseline	After reagent addition	Percentage change in discharge frequency （η/%）
PGE₂	6.6±2.3	2.1±2.6^*	-73.8±30.2
EP3 ant+PGE₂	6.2±2.2	6.1±2.2	-1.4±10.3^△

Group	Discharge frequency（Hz）		Percent change in discharge frequency （η/%）
Group	Baseline	After reagent addition	Percent change in discharge frequency （η/%）
PGE₂	7.8±6.5	3.6±7.1^*	-72.7±30.1
EP1 ant+PGE₂	8.1±7.0	3.6±7.6^*	-76.2±35.1

Group	Discharge frequency（Hz）		Percentage change in discharge frequency （η/%）
Group	Baseline	After reagent addition	Percentage change in discharge frequency （η/%）
PGE₂	7.6±2.1	2.1±2.4^*	-75.2±24.4
EP4 ant+PGE₂	7.3±3.6	2.1±2.6^*	-74.4±29.8

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Effect of prostaglandin E2 on discharge activity of warm-sensitive neurons in median preoptic nucleus of hypothalamus in female mice and its mechanism

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