Abstract:

o determine the effects of silibinin(SB) on the proliferation  and apoptosis of human bladder cancer T24 and 5637 cell lines,and to clarify the possible mechanism.
Methods Human bladder cancer 5637 and T24 cell lines  were cultured in cell media and the  5637 and T24 cells at logarithmic growth phase were divided into control group(0 μmol•L^-1  SB) and 25,50,100,200,and 400  μmol•L^-1  SB groups.The inhibitory effects of SB on the proliferation  of T24 and 5637 cells were  detected by  MTT assay;inverted phase contrast microscope was used to observe the invasion inhibition ability of T24 and 5637 cell lines after treated with different contentations of SB;the morphological changes of T24 and 5637 cells after treatment were observed by DAPI staining;the apoptosis   was detected and evaluated by flow cytometry.Results The  MTT results showed that  the  viability rates of  T24 and 5637 cell lines were decreased    in different concentrations of SB groups in a time- and concentration-dependent  manner compared with  control group.After treated with different concentrations of SB,the migration of T24 and 5637 cell lines was significantly inhibited.The DAPI staining and flow cytometry results revealed that the apoptotic rates of T24 and 5637 cells  in different concentrations of SB groups were higher than those in control group(P<0.05).Conclusion SB can  inhibit the proliferation and invasion of human bladder cancer T24 and 5637 cell lines  through inducing the apoptosis.

Key words: silibinin, bladder neoplasms, cell proliferation, apoptosis