The lung cancer H1299 cells were divided into control group （transfected with empty plasmid） and PDGF-D group （transfected with GV230-PDGF-D plasmid）； meanwhile，blank group was set up.Real-time fluorescence quantitative PCR（RT-qPCR） and Western blotting methods were used to detect the expression levels of PDGF-D mRNA and the expression amounts of PDGF-D protein in the cells in various groups.The H1299 cells were divided into control group（transfected with empty vector），PDGF-D group（transfected with GV230-PDGF-D plasmid），PDGF-D+PD98059（transfected with GV230-PDGF-D plasmid and treated with PD98059，an inhibitor of ERK）， and PD98059 group；the final concentration of PD98059 was 10 μmol·L^－1. MTT assay was used to detect the proliferation activities of cells in various groups. The migration rates of cells in various groups were detected by scratch test.The number of invasion cells in various groups was detected by Transwell assay. The expression levels of phosphorylated signal extracellular regulatory enzyme （p-ERK）， extracellular signal regulatory enzyme （ERK）， zinc finger transcription factor Snail，matrix metalloproteinases-1 （MMP-1） and transmembrane adhesion glycoproteins CD44 proteins in various groups were detected by Western blotting method.

The results of RT-qPCR method showed that compared with blank group and control group， the expression levels of PDGF-D mRNA in the cells in PDGF-D group were significantly increased （P<0.01）. The Western blotting results showed that there was no PDGF-D protein expression in the cells in blank group and control group；compared with blank group and control group，the expression amount of PDGF-D protein in PDGF-D group was siginificantly increased.Compared with control group， the proliferation activity of the cells and the migration rate in PDGF-D were significantly increased （P<0.05 or P<0.01）， the number of invasion cells was significantly increased （P<0.01）， and the expression levels of p-ERK， Snail， MMP-1 and CD44 proteins in the cells were increased （P<0.05）.Compared with PDGF-D group， the proliferation activity of the cells and the migration rate in PDGF-D+PD98059 group were significantly decreased （P<0.05），the number of invasion cells was decreased （P<0.05），and the expression levels of p-ERK， Snail， MMP-1 and CD44 proteins in the cells were decreased （P<0.05）.Compared with PDGF-D+PD98059 group， the proliferation activity of the cells and the migration rate in PD98059 group were significantly decreased （P<0.05 or P<0.01）， the number of invasion cells was decreased （P<0.01）， and the expression levels of p-ERK， Snail， MMP-1 and CD44 proteins in the cells were decreased （P<0.05 or P<0.01）.

PDGF-D can promote the proliferation， migration and invasion of lung cancer H1299 cells， and their mechanisms may be related to the up-regulation of Snail， MMP-1 and CD44 protein expressions through ERK signaling pathway.