Abstract:

Abstract:Objective
To discuss the anti-lipopolysaccharide(LPS) effect of the traditional antibacterial Chinese medicine Yinhuamiyanling Tablet,and to provide theoretical basis for its application. Methods Yinhuamiyanling Tablet were regarded as the study object,and the limulus test method was used to detect the anti-lipopolysaccharide activity.In vivo tests,90 ICR mice were divided into blank control group,drug control group,LPS control group,and low dose,middle dose,high dose prevention and treatment groups,and there were 10 mice in each group.The mice in low,middle,and high dose prevention and treatment groups were respectively fed with low dose (0.4 g/mL),middle dose (0.5 g/mL),high dose (0.6 g/mL) of Yinhuamiyanling Tablet solution.The mice in drug control group were fed with 0.6 g/mL  Yinhuamiyanling Tablet solution,and the mice in LPS control group and blank control group were fed with an equal volume of saline.In addition to  blank control group and drug control group,the mice in different prevention groups (3 h after the last administration) and different treatmentgroups(3 h before the first  administration) and LPS control group were intraperitoneally injected with 18 mg/kg LPS solution.48 h after LPS injection,the changes of body weight,blood parameters and organ indexes of the  mice in each group were observed.
Results The limulus test in vitro results showed that Yinhuamiyanling Tablet solution had anti-bacterial LPS activity even though its concentration was diluted to 6.25 g/L from 100 g/L.In vivo, compared with blank control group,the body weight,the number of white blood cells (WBC),red blood cells (RBC),concentration of haemoglobin(Hb),hematocrit(Hct),platelet(PLT) of the mice in LPS control group were significantly decreased (P<0.05 or P<0.01);the spleen indexes and lung indexes were significant increased(P<0.01).Compared with LPS control group,the body weight of the mice in  low dose treatment group and middle dose prevention group were significantly increased 24 h after LPS injection（P<0.05）;the body weight of the mice in  middle dose and high dose prevention groups were significantly increased 36 h after LPS injection（P<0.05）;the body weight of the mice in middle dose prevention groups was significantly increased 72 h after LPS injection（P<0.05）；the number of WBC of the mice in high dose treatment group was significantly increased (P<0.05),the number of RBC of the  mice  in low dose treatment group and middle dose prevention group was significantly increased  (P<0.01);the number of  Hb of the mice in low dose treatment group and different prevention groups was significantly increased (P<0.05 or P<0.01);the number of Hct of the  mice in low dose and high dose prevention groups was significantly increased (P<0.05 or P<0.01);the number of PLT of the mice in low and  high dose prevention groups was significantly increased  (P<0.05);the liver index of the  mice in  middle dose treatment group was significantly decreased (P<0.01);the lung index of the mice in high dose treatment group was significantly decreased (P<0.05);the spleen indexes of the mice in low dose,high dose treatment groups and different  prevention groups was significantly deceased (P<0.05 or P<0.01).Conclusion Yinhuamiyanling Tablet has anti-bacterial LPS activity in vitro and in vivo.

Key words: Yinhuamiyanling Tablet, lipopolysaccharide, limuls test, blood parameter, organ index