Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (01): 10-15.doi: 10.13481/j.1671-587x.20150103

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Role of TLR subunits 2 and 4 in pathogenesis of allergic rhinitis in rats

ZHANG Min1, LIU Lixing1, HU Jianzhang2   

  1. 1. Department of Physiology, Basic Medical College, Putian University, Putian 351100, China;
    2. Department of Ophthalmology, First Affiliated Hospital, Fujian Medical University, Fuzhou 350001, China
  • Received:2014-01-03 Published:2015-01-30

Abstract:

Objective To build the rat allergic rhinitis(AR) model with OVA, and to investigate the role of TLR subunits 2 and 4 in the pathogenesis of AR in rats. Methods 80 rats were randomly divided into normal group, model group, AR+LPS group and AR+PGN group(n=20).The morphological changes of nasal mucosa tissue and the number of inflammatory cells and infiltration were observed by HE staining.The expression levels of TLR2, TLR4 and IgE in nasal mucosa tissue were measured with immunohistochemical method.Realtime-PCR was used to evaluate the expression levels of TLR2 mRNA and TLR4 mRNA in the nasal mucosa tissue of the rats in various groups. Results Compared with normal control group, the scores of nasal symptoms and the number of inflammatory cells in model group, AR+LPS group and AR+PGN group were significantly increased(P<0.05).Compared with normal group, the allergic injury of nasal mucosa of the rats in model group was obvious;the eosinophil count and the expression levels of TLR2, TLR4 and IgE in model group were significantly increased(P<0.05), while the neutrophil count was significantly increased in LPS group(P<0.05).Compared with model group, the expression levels of TLR4 and IgE in AR+LPS group were significantly increased (P<0.05), and the expression levels of TLR2 and IgE in AR+PGN group were significantly increased (P<0.05). Conclusion Intraperitoneal injection and local nasal of OVA could effectively build AR model;TLR2 and TLR4 play an important role in the pathogenesis of AR.

Key words: allergic rhinitis, Toll-liked receptor subunits, lipopolysaccharide, peptidoglyvans

CLC Number: 

  • R765.21