克林霉素-PLGA微球的制备及其体外抑菌活性评价

doi:10.13481/j.1671-587x.20150118

Abstract

Abstract:

Objective To prepare the clindamycin-loaded sustained release microspheres with poly (lactic-co-glycolic acid) (PLGA) which have good biodegradability, and to investigate its related properties. Methods The clindamycin-loaded PLGA microsheres were prepared by emulsion-solvent evaporation method. Then the morphology and particle size distribution of clindamylin-PLGA microsheres were detected. And two factors affecting entrapment rate of microspheres, including the rate of PLGA/dichloromethane(P/D) and PLGA/clindamycin (P/C), were investigated.In addition, the clindamycin-PLGA was dispersed in dissolve medium and oscillated at 37℃.Then the solution at different time was taken.Kirby-Bauer method was used to observe the ability of bacteriostasis by measuring the bacteriostatic ring size at different time. Results The acquired PLGA microspheres containing drugs exhibited well-defined properties, with the even and uniform sphere in appearance, general particles without adhesion, at the mean diameter of 700 nm mostly. The optimal ratio of P/D was 80/1 and P/C was 5/1, and the entrapment rate obtained was 45.02%.Moreover the antibacterial effect was very obvious on the initial release, followed by a prolonged release from days 3 to 19.The minimum inhibitory concentration (MIC) was reached on day 20. Conclusion Clindamycin-PLGA microspheres with good morphology and high encapsulation efficiency can be obtained using optimized formulation.The microspheres have a good antimicrobial effect in vivo through a long period of sustained release of drugs.

Key words: clindamycin, poly (lactic-co-glycolic acid), microspheres, antibacterial activity, sustained release

CLC Number:

R782.3

LIN Tao, LI Minghe, HAO Ying, MAO Jixiong, BA Te, HAN Chengmin. Preparation of clindamycin-loaded PLGA microspheres and evaluation on its in vitro antibacterial activity[J].Journal of Jilin University Medicine Edition, 2015, 41(01): 94-98.

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Preparation of clindamycin-loaded PLGA microspheres and evaluation on its in vitro antibacterial activity

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