Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (01): 114-119.doi: 10.13481/j.1671-587x.20150122

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Expression of STK15 in head and neck squamous cell carcinoma tissue and its effect on growth of Hep-2 cells

LI Yadong, ZHANG Jinsong   

  1. Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Received:2014-07-31 Published:2015-01-30

Abstract:

Objective To expore the expression of serine/threonine kinase 15 gene (STK15) in head and neck squamous cell carcinoma(HNSCC)tissue and the relationship with the clinicopathological characteristics of HNSCC, and to clarify the effect of silencing STK15 on Hep-2 cells. Methods Immunohistochemistry was used to detect the expression of STK15 protein in the HNSCC tissue.Lentiviral silencing STK15 was used in Hep-2 cell line to obtain stable transfection cell line.The Hep-2 cells were divided into control group(PLKO.1-puro-siRNA)and experimental group(PLKO.1-puro-siSTK15).Western blotting method, RT-PCR, MTT assay, cell migration assay and immunofluorescence assay were used to study the expression of STK15 protein and STK15 mRNA, cell proliferation, migration and the number of centrosome. Results Immunohistochemical staining revealed that the expression rate of STK15 protein in HNSCC tissue was 64.96%, and the expression of STK15 protein was correlated with TNM stage and prognosis(P<0.05). The results of Western blotting method showed the expression level of STK15 protein in experimental group was lower than that in control group(P<0.05).The results of RT-PCR showed the expression level of STK15 mRNA in experimental group was lower than that in control group(P<0.05).The results of proliferation assay showed the proliferation rate of the cells in experimental group was lower than that in control group(P<0.05).The results of cell migration assay showed the percentage of the cells penetrating the membrane in experimental group was lower than that that in control group(P<0.05).The results of immunofluorescence assay showed that the number of centrosome in experimental group was lower than that in control group(P<0.05). Conclusion The expression of STK15 protein is closely correlated with the development and prognosis of HNSCC and silencing STK15 can greatly reduce the proliferation and migration ability of Hep-2 cells.STK15 could represent a potentially new important therapeutic target of HNSCC, and the status of STK15 expression may also become a novel and prognostic biomarker for HNSCC patients.

Key words: serine/threonine kinase 15 gene, head and neck neoplasms, prognosis

CLC Number: 

  • R939.9