邻苯二甲酸二丁酯对雄性小鼠生殖系统的脂质过氧化作用

doi:10.13481/j.1671-587x.20150323

Abstract

Abstract:

Objective To observe the lipid peroxidation of dibutylphthalate(DBP) on the reproductive system of the male mice and its influence in the antioxidant enzyme activities, and to investigate the possible mechanism of DBP to the reproductive system of the male mice. Methods 40 healthy male ICR mice were randomly divided into control group, 200 mg·kg^-1 DBP group, 400 mg·kg^-1 DBP group, and 800 mg·kg^-1 DBP group(n=10).The mice in DBP groups were administrated with 200, 400 and 800 mg·kg^-1·d^-1 DBP by gavage.The mice in control group were only received corn solvent.5 mice in each group were sacrificed respectively 2 and 4 weeks later, and their testis were collected.The malondialdehyde (MDA) levels, activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in testis tissue of the mice in various groups were detected. Results Two weeks after exposure, the MDA levels in testis tissue of the mice in 400 and 800 mg·kg^-1 DBP groups were significantly higher than those in control group and 200 mg·kg^-1 DBP group(P<0.05);the SOD activities in 400 and 800 mg·kg^-1 DBP groups were lower than that in 200 mg·kg^-1 DBP group (P<0.05).Four weeks after exposure, the testis organ coefficients in 400 and 800 mg·kg^-1 DBP groups were lower than those in control group and 200 mg·kg^-1DBP group (P<0.05);the MDA levels in 400 and 800 mg·kg^-1 DBP groups were higher than that in control group (P<0.05), and the MDA level in 200 mg·kg^-1 DBP group was lower than that in control group (P<0.05).The SOD activity in testis tissue of the mice in 800 mg·kg^-1DBP group was significantly lower than those in control group, 200 mg·kg^-1 DBP group and 400 mg·kg^-1 DBP group (P<0.05);the GSH-Px activities in testis tissue of the mice in DBP groups were significantly higher than that in control group (P<0.05). Conclusion DBP can induce the lipid peroxidation of the mouse testis and induce oxidative stress, and lead to the descending of testicular antioxidant capacity, then Results in reproductive dysfunction.

Key words: dibutylphthalate, malondialdehyde, glutathione peroxidase, superoxide dismutase, lipid pexoxidation

CLC Number:

R114

WU Guanyu, WANG Shuo, LIU Yan, ZHAO Shuhua. Lipid peroxidation of dibutylphthalate on reproductive system of male mice[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 553-557.

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Lipid peroxidation of dibutylphthalate on reproductive system of male mice

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