OX-LDL介导内皮细胞损伤及其对TLR-4和EpCAM表达的影响

doi:10.13481/j.1671-587x.20150609

Abstract

Abstract:

Objective To study the damage effect of oxidized low density lipoprotein (OX-LDL) on the endothelial cells and its influence in the expressions of Toll like receptor 4 (TLR-4) and epithelial cell adhesion molecule(EpCAM). Methods The human endothelial cells ECV-304 were cultured in vitro and treated by different concentrations of OX-LDL for 24 h, and the cell vitality was measured by Taipan blue rejection test.The reactive oxygen species(ROS) level,mitochondrial membrane potential(MMP),cell cycle and apoptosis were detected by flow cytometry (FCM).The expression of TLR-4 and EpCAM were also analyzed by FCM. Results Compared with control group, the ROS levels in ECV-304 cells in 25,50,100, and 200 mg·L^-1 OX-LDL were increased,but the cell vitalities and MMP were decreased(P<0.05). Compared with control group, the percentages of S phase cells in 25 and 50 mg·L^-1 OX-LDL groups were increased,the G₀G₁ phase cells were slightly reduced, and the SubG₁ phase cells (apoptotic cells) were increased with the increasing of OX-LDL concentration. Compared with control group, the TLR-4 and EpCAM expression levels in ECV-304 cells in 50 mg·L^-1 OX-LDL group at 24 h were significantly increased (P<0.05). Conclusion OX-LDL could increase the ROS level and induce the apoptosis in vascular endothelial cells,while increase the cell vitality and MMP.During the damage process, the expresion levels of TLR-4 and EpCAM are up-regulated.

Key words: oxidized low density lipoprotein, vascular endothelial cell, reactive oxygen species, epithelial cell adhesion molecule, Toll like receptor-4

CLC Number:

R541.6

CHEN Wei, ZHANG Kui, ZHANG Chenhao, LI Qiang, LI Yan. Damage of endothelial cells induced by OX-LDL and its influence in expressions of TLR-4 and EpCAM[J].Journal of Jilin University Medicine Edition, 2015, 41(06): 1144-1149.

References

[1] Onat D, Brillon D, Colombo PC, et al. Human vascular endothelial cells: a model system for studying vascular inflammation in diabetes and atherosclerosis[J].Curr Diab Rep,2011,11(3):193-202.

[2] Park C, Kim TM, Malik AB.Transcriptional regulation on endothelial cell and vascular development[J].Circ Res,2013,112(10): 1380-1400.

[3] Sima AV, Botez GM, Stancu CS,et al. Effect of irreversibly glycated LDL in human vascular smooth muscle cells: lipid loading, oxidative and inflammatory stress [J]. Cell Mol Med, 2010,14(12):2790-2802.

[4] Merino A, Portolés J, Selgas R, et al. Effect of different dialysis modalities on microinflammatory status and endothelial damage[J].Clin J Am SocNephrol,2010,5(2):227-234.

[5] Kranidioti H, Orfanos SE, Vaki I, et al. Angiopoietin-2 is increased in septic shock: evidence for the existence of a circulating factor stimulating its release from human monocytes [J].Immunol Lett, 2009,125(1):65-71.

[6] Wu CJ, Mannan P, Lu M,et al. Epithelial cell adhesion molecule (EpCAM) regulates claudin dynamics and tight junctions[J]. J Biol Chem, 2013,288(17):12253-12268.

[7] Wang X, Wang Y, Yang M, et al. A newly identified epithelial cell adhesion molecule (EpCAM) from grass carp (Ctenopharyngodon idellus): cloning, tissue distribution and lipopolysaccharide-induced expression in head kidney leucocytes [J].Fish Shellfish Immunol, 2010,29(6):998-1002.

[8] Di Pietro M, Filardo S, De Santis F, et al. Chlamydia pneumoniae and oxidative stress in cardiovascular disease: state of the art and prevention strategies [J]. Int J Mol Sci, 2014,16(1):724-735.

[9] Lee WJ, Ou HC, Hsu WC, et al.Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells [J]. Vasc Surg, 2010,52(5):1290-1300.

[10] 冉小丽,吴芹,石京山,等.凝集素样氧化型低密度脂蛋白受体1在动脉粥样硬化中的作用[J].中国药理学与毒理学杂志,2013,27(5):865-869.

[11] Li C, Zhao G, Che C, et al. The role of LOX-1 in innate immunity to aspergillus fumigatus in corneal epithelial cells[J].Invest Ophthalmol Vis Sci,2015,56(6):3593-3603.

[12] Feng Y,Cai ZR, Tang Y, et al.TLR4/NF-κB signaling pathway-mediated and oxLDL-induced up-regulation of LOX-1, MCP-1, and VCAM-1 expressions in human umbilical vein endothelial cells[J]. Genet Mol Res,2014,13(1):680-695.

[13] Schilling JD, Machkovech HM, He L, et al. TLR4 activation under lipotoxic conditions leads to synergistic macrophage cell death through a TRIF-dependent pathway [J]. J Immunol, 2013,190(3):1285-1296.

[14] 张伟, 蒋耀光, 谢志坚,等.严重胸部创伤复合内毒素感染对肺泡与肺间质巨噬细胞吞噬功能的影响[J].中国危重病急救医学,2003,15(7): 408-410.

[15] 刘铭,王志禄.肾素-血管紧张素-醛固酮系统及其受体与心血管疾病[J].中华心血管病杂志,2002,30(3):190-192.

[16] 于振香, 赵珩, 彭丽萍.人参二醇组皂苷对失血-内毒素二次打击大鼠肺脏氧化损伤的保护作用[J].吉林大学学报:医学版,2008,34(4):594-598.

[17] Karnati HK, Pasupuleti SR, Kandi R,et al. TLR-4 signalling pathway: MyD88 independent pathway up-regulation in chicken breeds upon LPS treatment [J]. Vet Res Commun, 2015,39(1):73-78.

[18] Ni J, Cozzi P, Hao J, et al. Epithelial cell adhesion molecule (EpCAM) is associated with prostate cancer metastasis and chemo/radioresistance via the PI3K/Akt/mTOR signaling pathway [J]. Int J Biochem Cell Biol, 2013,45(12):2736-2748.

Damage of endothelial cells induced by OX-LDL and its influence in expressions of TLR-4 and EpCAM

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