黄芪甲苷对糖尿病肾脏病变大鼠氧自由基代谢和转化生长因子β1mRNA表达的影响

doi:10.13481/j.1671-587x.20160110

Abstract

Abstract:

Objective: To study the effect of astragaloside on the oxygen radical metabolism and (DN)the expression of transforming grow th factor β1(TGF-β1)mRNA in the rats with STZ-induced diabetic nephropathy(DN), and to clarify its related mechanism. Methods: 60 Wistar rats were divided into control group,model group,irbesartan group,low dose (0.75 mg·kg·d^-1)of astragaloside group,middle dose (1.50 mg·kg·d^-1)of astragaloside group and high dose (3.00 mg·kg·d^-1)of astragaloside group(n=10).Except control group,the rats in other groups were intraperitoneally injected with 60 mg·kg·d^-1 STZ to induce DN models.8 weeks later,the levels of serum creatinine(Scr),blood urea nitrogen(BUN),fasting blood glucose,and 24 h urinary protein of the rats in various groups were detected.The SOD and GSH-Px activities and MDA levels in kidney tissue of the rats in various groups were measured;the expression levels of CAT and TGF-β1 mRNA in kidney tissue were determined by RT-PCT. Results: Compared with model group,the kidney function of the DN rats in middle and high doses of astragaloside groups was improved;the serum Scr levels of the rats in astragloside groups were decreased (P<0.05 or P<0.01),and the levels of serum BUN and PBG and 24 h urinary protein of the rats in middle and high doses of astragaloside groups were decreased(P<0.05 or P<0.01).Compared with model group,the MDA levels in kidney tissue of the rats in middle and high doses of astragaloside groups were decreased and the SOD activites were increased(P<0.05 or P<0.01); the expression levels of TGF-β1 mRNA were decreased(P<0.05); the GSH activities in kidney tissue of the rats in various doses of astragaloside groups were increased P<0.05 or P<0.01); but the expression levels of CAT mRNA had no changes(P>0.05). Conclusion: Astragaloside can improve the kidney function of DN rats,its mechanism may be related to the improvement of oxygen radical damage and reduction of TGF-β1 mRNA expression.

Key words: astragaloside, diabetic nephropathy, oxidative stress, reactive oxygen species, transforming growth factor β1

CLC Number:

R285.5

HUANG Xiaodong, SHEN Nan, LU Qian, LIU Wei, CHANG Ying, FAN Hongyan, WANG Yanchun. Effect of astragaloside on oxygen radical metabolism and expression of TGF-β1 mRNA in diabetic nephropathic rats[J].Journal of Jilin University Medicine Edition, 2016, 42(01): 48-53.

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Effect of astragaloside on oxygen radical metabolism and expression of TGF-β1 mRNA in diabetic nephropathic rats

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