Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (02): 306-310.doi: 10.13481/j.1671-587x.20160222

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Application of combined detection of serum levels of ADA and T-spot.TB in diagnosis of pulmonary tuberculosis

FENG Xiaomeng, WANG Jiingying, SHI Jingwei, LI Wei, GAO Shen, FENG Xuefei, XIE Feng   

  1. Deparment of Clinical Laboratory, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2015-11-09 Published:2016-03-31

Abstract:

Objective: To combine the detection of serum levels of adenosine deaminase(ADA)and T-cell spot test(T-spot.TB), and to explore their significances in diagnosis of pulmonary tuberculosis.Methods: 159 patients suspected with pulmonary tuberculosis were selected and divided into pulmonary tuberculosis group and non-tuberculosis group(n=68); 80 healthy people were randonly selected as healthy control group. The serum ADA levels and number of T-spot of the subjects in three groups were detected. Ther serum ADA levels and the positive rates of T-spot.TB in various groups and their sensitivities and specifities were compared.Results: The serum ADA level of the patients in pulmonary tuberculosis gruop was (22.10±6.60)U·L-1;those in non-tuberculosis group and healthy control group were (16.90±6.35) and (8.70±5.98)U·L-1;the serum ADA level in pulmonary tuberculosis group was significantly higher than those in non-tuberculosis group and heathy control group(P<0.05).The positive rate of serum ADA level in diagnosis of pulmonary tuberculosis was 56% and the T-spot.TB positive rate in diagnosis of pulmonary tuberculosis was 87.9%.Combined use of parallel test, the detection sensitivity was 91.2%; using the series of joint tests, the specificity was 94.6%.Conclusion: Combined detection of serum level of ADA and T-spot.TB can significantly improve the clinical diagnosis efficacy of pulmonary tuberculosis.

Key words: pulmonary tuberculosis, adenosine deaminase, T-spot.TB

CLC Number: 

  • R52