传统中药联合标准三联疗法治疗幽门螺杆菌诱发的慢性非萎缩性胃炎及其作用机制

doi:10.13481/j.1671-587x.20160429

Abstract

Abstract:

Objective: To treat the chronic non-atrophic gastritis patients induced by Helicobacter pylori with Qingweizhitong Weiwan combined with standard triple therapy,and to detect the differential expression of related immflammation genes with PCR array, and to clarify its mechanism. Methods: Ten patients with chronic non-atrophic gastritis complicated with Helicobacter pylori infection were used as treatment group and 10 health people were used as health control group. The patients in treatment group were treated with Qingweizhitong Weiwan combined with standard triple therapy for 14 d. The blood samples of the subjects in treatment group and health control group were collected before and after treatment, and QIAGEN human antibacterial response PCR array was performed to test the total RNA inperipheral blood and to analyze the differential expressions of 84 inflammation-related genes. Results: The differential expressions of 20 inflammation-related genes were found. Compared with health control group, the expression levels of 20 genes in treatment group before treatment were up-regulated (Fold-change>2);after treatment, the expression levels of 20 genes were down-regulated, and 11 of them were similar to the level in health control group (Fold-change < 2). More specifically, part of 20 genes was related to NLRP3 inflammasome. Compared with health control group, the gene expression levels of CASP1, IL1B, NLRP3, and PYCARD in treatment group before treatment were up-regulated (P < 0.05).Compared with before treatment, the expression levels of CASP1, IL1B, NLRP3, and PYCARD in treatment group after treatment were down-regulated (P < 0.05). Conclusion: The mechanism of Qingweizhitong Weiwan combined with standard triple therapy in the treatment of chronic non-atrophic gastritis patients induced by Helicobacter pylori may be related to inhibiting the expressions of NLRP3 inflammasome-related genes and interfering the antimicrobial innate immune response.

Key words: PCR array, Qingweizhitong Weiwan, NLRP3 inflammasome, combination therapy

CLC Number:

R737.3

SONG Chenxue, WANG Yubo, LIU Chuangui, XIE Jingshu, LU Yanjiao, WANG Ting, WANG Guoqiang, WANG Yawei, WANG Fang, ZHENG Jingtong. Treatment of Helicobacter pylori induced chronic atrophic gastritiswith traditional Chinese medicine combined standard triple therapyand its mechanisms[J].Journal of Jilin University Medicine Edition, 2016, 42(04): 789-792.

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Treatment of Helicobacter pylori induced chronic atrophic gastritiswith traditional Chinese medicine combined standard triple therapyand its mechanisms

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