TGF-β1对胶质瘤U87细胞上皮-间质转化的诱导作用

doi:10.13481/j.1671-587x.20160609

Abstract

Abstract:

Objective: To investigate the induction of transforming growth factor beta 1 (TGF-β1) on epithelial to mesenchymal transition (EMT) in glioma U87 cells, and to provide evidence to clarify the mechanism of glioma invasion growth.Methods: The glioma U87 cells were treated with different concentrations of TGF-β1 in vitro and divided into 2.5×10^-6,5.0×10^-6,10.0×10^-6,and 20.0×10^-6g·L^-1 TGF-β1 groups,and 0×10^-6g·L^-1 TGF-β1 treatment was regarded as blank control group.The relative expression levels of epithelial marker(E-cadherin),mesenchymal markers(N-cadherin,Vimentin),and transcription factors(Snail,Slug, and Zeb1 proein) in the U87 cells in various groups were detected by Western blotting method; the morphology of U87 cells was observed by inverted microscope; the relative wound space of U87 cells was examined by scratch healing assay.Results: With the increasing of concentrations of TGF-β1,the relative expression levels of E-cadherin in glioma U87 cells in 2.5×10^-6,5.0×10^-6,10.0×10^-6, and 20.0×10^-6 g·L^-1 groups were gradually decreased compared with blank control group (P < 0.05 or P < 0.01).The relative expression levels of N-cadherin and Vimentin showed an increasing trend,and the relative expression levels of N-cadherin were increased by 11.5%(P>0.05),39.4%(P>0.05),51.0%(P<0.05), and 76.9%(P<0.05) in 2.5×10^-6,5.0×10^-6,10.0×10^-6, and 20.0×10^-6 g·L^-1 TGF-β1 groups compared with blank control groups. The relative expression levels of Vimentin in the U87 cells in TGF-β1 10×10^-6 and 20×10^-6g·L^-1 groups were higher than those in blank control group(P<0.01). The relative expression levels of Snail,Slug and Zeb1 proteins in the U87 cells in 5.0×10^-6,10.0×10^-6, and 20.0×10^-6g·L^-1 groups were higher than those in blank control group(P < 0.05 or P < 0.01). Compared with blank control group,the morphology of U87 cells in 10×10^-6g·L^-1TGF-β1 group became more stretching,long and narrow, and the cell migration ability was also significantly enhanced.Conclusion: Within a certain concentration range,TGF-β1 could induce EMT in glioma U87 cells in a concentration-dependent manner.

Key words: transforming growth factor beta 1, epithelial to mesenchymal transition, cadherin, transcription factor, glioma cell

CLC Number:

R739.4

SONG Yang, ZHAO Liyan, CHEN Yong, LYU Xiaoyan, CUI Jiayue, LI Yunqian, ZHAO Gang. Induction of TGF-β1 on epithelial to mesenchymal transition in glioma U87 cells[J].Journal of Jilin University Medicine Edition, 2016, 42(06): 1087-1091.

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Induction of TGF-β1 on epithelial to mesenchymal transition in glioma U87 cells

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