非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响

doi:10.13481/j.1671-587x.20170102

Abstract

Abstract:

Objective: To study the influence of endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA) in the electrical activity of pacemaker cells in the guinea-pig left ventricular outflow tract,and to explore the effect of ADMA on the occurrence and development of ventricular outflow tract arrhythmias.Methods: The isolated guinea-pig left ventricular outflow tract tissue was perfused with fully oxygenated Locke solution under constant temperature and constant flow velocity.The spontaneous slow action potentials(sAP) of guinea-pig left ventricular tract were recorded by conventional intracellular microelectrode technique.The effects of NOS inhibitor ADMA(1,10 and 100 μmol·L^-1) on the spontaneous sAP were studied.And the effects of ADMA under the condition of pretreatment of sodium nitroprusside(SNP) were also explored(control group,10 μmol·L^-1 SNP group,and SNP+ADMA group).Results: Compared with control group,the different indexes of sAP in 1 μmol·L^-1 ADMA group had no significant changes(P>0.05).Compared with control group, the velocity of diastolic depolarization (VDD),the rate of pacemaker firing (RPF) and the maximal rate of depolarization(V_max) in 10 and 100 μmol·L^-1 ADMA groups were siginificantly decreased(P<0.05),and the 50% and 90% of duration of action potential(APD₅₀ and APD₉₀) were markedly increased(P<0.05). Compared with 1 μmol·L^-1ADMA group,the RPF in 10 μmol·L^-1 ADMA group was siginificantly decreased(P<0.05) and the APD₉₀ was markedly increased(P<0.05); compared with 1 μmol·L^-1ADMA group,the RPE,V_max,and APD₅₀ had significant changes (P<0.05).Compared with 10 μmol·L^-1ADMA group,the APA in 100 μmol·L^-1ADMA group was significantly decreased (P<0.05). After pretreatment of 10 μmol·L^-1 SNP for 10 min,followed by 10 μmol·L^-1 SNP and 10 μmol·L^-1 ADMA,the inhibitory effects of spontaneous activity induced by ADMA could be reversed;compared with control group,the VDD and RPF were significantly increased (P<0.05),the V_max and APA had no significant changes(P>0.05), and the APD₉₀ was markedly decreased (P<0.05).Conclusion: Endogenous NOS inhibitor ADMA can decrease the spontaneous activity of left ventricular outflow tract,and the inhibitory effects induced by ADMA can be reversed under the condition of pretreatment of NO donor SNP.

Key words: left ventricular outflow tract, spontaneous slow action potentials, asymmetric dimethylarginine

CLC Number:

Q463

ZHAO Lanping, XUE Shufang, CHEN Yanjing, WANG Xuefang, CHEN Lifeng, HUANG Jiacheng. Influence of ADMA in electrical activity of pacemaker cells in guinea-pig left ventricular outflow tract[J].Journal of Jilin University Medicine Edition, 2017, 43(01): 6-10.

References

[1] 陈彦静,葛赋贵.豚鼠主动脉前庭组织的自律性研究和形态学观察[J].生理学报,1994,46(5):458-464.
[2] 张晓云,陈彦静,葛赋贵,等.兔主动脉前庭自律细胞与窦房结电生理特性的比较[J].生理学报,2003,55(4):405-410.
[3] 张慧平, 柳景华.非对称性二甲基精氨酸与冠心病的相关性研究进展[J].中国临床保健杂志,2014,17(6):667-670.
[4] 黄新金,李菊香,颜素娟,等.冠状微血管痉挛性心绞痛患者血内源性一氧化氮合酶抑制物的变化[J].中国循环杂志,2007,22(2):100-102.
[5] 王亮,张抒扬.新的血管内皮损伤标记物-非对称性二甲基精氨酸[J].中华临床医师杂志:电子版,2011,5(16):4795-4797.
[6] Zhao JR,Zhang DY,Sun DZ.Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients[J].Int J Chin Exp Med,2014,7(11):4455-4460.
[7] Rafat P,Pawet G,Matgorzata P,et al.Left ventricular diastolic dysfunction and plasma asymmetric dimethylarginine concentration in patients with essential hypertension[J].Arch Med Sci,2015,11(3):521-529.
[8] Saliba WI,Natale A.Ventricular tachycardia syndromes[J]. Med Clin North Am,2001,85(2):267-304.
[9] 王洪巨,刘俊,史晓俊,等.原发性高血压患者血浆非对称性二甲基精氨酸浓度升高[J].中华高血压杂志,2007,15(11):930-932.
[10] BögerRH.Asymmetric dimethylarginine,an endogenous inhibitor of nitric oxide synthase,explains the "L-arginine paradox" and acts as a novel cardiovascular risk factor[J].J Nutr,2004,134(10 suppl):2842s-2847s.
[11] Ferreira-Melo SE,Demacq C,Lacchini S,et al.Sildenafil preserves diastolic relaxation after reduction by L-NAME and increases phosphodiesterase-5 in the intercalated discs of cardiac myocytes and arterioles[J].Clinics (Sao Paulo),2011,66(7):1253-1258.
[12] Luo Y,Cha DG,Liu YL,et al.Differential effects of selective and non-selective nitric oxide synthase inhibitors on the blood perfusion of ischemia-reperfused myocardium in dogs[J].Med Sci Monit Basic Res,2013,19:181-186.
[13] 聂凡,宜全,张贵平,等.一氧化氮合酶抑制物对大鼠离体乳头肌收缩力的影响及其机制[J].国际病理科学与临床杂志,2012,32(1):1-7.
[14] Wang LX. Role of nitric oxide in regulating cardiac electrophysiology[J].Exp Clin Cardiol,2001,6(3):167-171.
[15] Bonilla IM,Sridhar A,Györke S,et al.Nitric oxide synthases and atrial fibrillation[J].Front Physiol,2012,3:105.
[16] 赵兰平,薛淑芳,陈彦静,等.一氧化氮对缺血再灌注豚鼠左心室流出道自律性电活动的影响[J].中国病理生理杂志,2015,31(7):1166-1171.

Influence of ADMA in electrical activity of pacemaker cells in guinea-pig left ventricular outflow tract

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