人生长激素释放激素受体剪接变异体1型对人肝癌细胞HepG2增殖的影响

doi:10.13481/j.1671-587x.20170303

Abstract

Abstract: Objective: To investigate the effect of human growth hormone releasing hormone receptor splice variant type 1 (GHRHR SV1) on the proliferation of human liver cancer HepG2 cells, and to clarify the proliferation effect of GHRHR SV1 on the human cancer cells. Methods: The GHRHR SV1 plasmids were transfected into the human HepG2 cells to construct the HepG2-SV1 cell line. HepG2 group(HepG2 cells),HepG2-empty group(HepG2-pcDNA3.0 cell line) and HepG2-SV1 group(HepG2-SV1 cells) were set up. PCR and Western blotting methods were used to identify the HepG2-SV1 cell line;CCK-8 method was used to detect prolifernation rate of cells;colony formation assay was used to detect the colony formation rate of cells;cell wound healing assay was used to evaluate the migration rate of cells. Results: The PCR and Western blotting results showed the HepG2-SV1 cell line expressed GHRHR SV1 steadily.The CCK-8 results showed that the proliferation rate of the HepG2-SV1 cells in HepG2-SV1 group was higher than that of the HepG2-pcDNA3.0 cells in HepG2-empty group(P<0.05).The colony formation assay results showed that the colony formation rate of HepG2-SV1 cells in HepG2-SV1 group was 3.5 times higher than that of the HepG2-pcDNA3.0 cells in HepG2-empty group(P<0.05).The cell wound scratch assay results showed that the migration rate of the HepG2-SV1 cells in HepG2-SV1 group was higher than that of the HepG2-pcDNA3.0 cells in HepG2-empty group(P<0.05). Conclusion: GHRHR SV1 could increase the proliferation of HepG2 cells.

Key words: HepG2 cells, cell proliferation, colony formation rate, migration rate, human growth hormone releasing hormone receptor splice variant type 1

CLC Number:

R735.7

LIN Yuyin, CHEN Wensheng, GUO Xiaolan, TAN Yin, HE Xiaosong, DAI Jianwei. Effect of human growth hormone releasing hormone receptor splice variant type 1 on proliferation of human liver cancer HepG2 cells[J].Journal of Jilin University Medicine Edition, 2017, 43(03): 474-478.

References

[1] Hohla F,Moder A,Mayrhauser U,et al.Differential expression of GHRH receptor and its splice variant 1 in human normal and malignant mucosa of the oesophagus and colon[J].Int J Oncol,2008,33(1):137-143.
[2] Papadia A,Schally AV,Halmos G,et al.Growth hormone-releasing hormone antagonists inhibit growth of human ovarian cancer[J].Horm Metab Res,2011,43(11):816-820.
[3] Dioufa M,Farmaki E,Schally AV,et al.Growth hormone-releasing hormone receptor splice variant 1 is frequently expressed in oral squamous cell carcinomas[J].Horm Cancer,2012,3(4):172-180.
[4] Chatzistamou I,Volakaki A,Schally AV,et al.Expression of growth hormone-releasing hormone receptor splice variant 1 in primary human melanomas[J].Regul Pept,2008,147(1-3):33-36.
[5] Mezey G,Treszl A,Schally AV,et al.Prognosis in human glioblastoma based on expression of ligand growth hormone-releasing hormone,pituitary-type growth hormone-releasing hormone receptor,its splicing variant receptors,EGF receptor and PTEN genes[J].J Cancer Res Clin Oncol,2014,140(10):1641-1649.
[6] 郭晓兰,谭茵,陈文生,等.GHRHR-SV1抗体的制备及其蛋白在黑色素瘤细胞中的表达[J].吉林大学学报:医学版,2016,42(6):1237-1242.
[7] 田力,张蕾,李伟伟,等.人生长激素释放激素变异受体1型基因绿色荧光蛋白真核表达载体1的构建及体外表达[J].江西医学学报,2009,49(3):15-20.
[8] 汪期明,黄勇.生长激素释放激素受体剪接变异体1在子宫内膜异位症中的表达[J].中国妇幼保健,2012,27(28):4441-4444.
[9] 沈烨,胡吉,张玉林,等.生长激素释放激素在垂体生长激素瘤中的作用[J].复旦学报:医学版,2006,33(4):463-466.
[10] 黄勇,汪期明.生长激素释放激素拮抗剂对人异位子宫内膜抑制增殖作用与cAMP信号通路相关[J].医学研究杂志,2013(5),103-106.
[11] Szalontay L,Schally AV,Popovics P,et al.Novel GHRH antagonists suppress the growth of human malignant melanoma by restoring nuclear p27 function[J].Cell Cycle,2014,13(17):2790-2797.
[12] Munoz-Moreno L,Arenas MI,Schally AV,et al.Inhibitory effects of antagonists of growth hormone-releasing hormone on growth and invasiveness of PC3 human prostate cancer[J].Int J Cancer,2013,132(4):755-765.
[13] Kovacs M,Schally AV,Hohla F,et al.A correlation of endocrine and anticancer effects of some antagonists of GHRH[J].Peptides,2010,31(10):1839-1846.
[14] Munoz-Moreno L, Arenas MI, Carmena MJ, et al.Growth hormone-releasing hormone antagonists abolish the transactivation of human epidermal growth factor receptors in advanced prostate cancer models[J].Invest New Drugs,2014,32(5):871-882.
[15] Rick FG,Schally AV,Szalontay L,et al.Antagonists of growth hormone-releasing hormone inhibit growth of androgen-independent prostate cancer through inactivation of ERK and Akt kinases[J].Proc Natl Acad Sci USA,2012,109(5):1655-1660.
[16] Catanuto P,Tashiro J,Rick F,et al.Expression of receptors for pituitary-type growth hormone-releasing hormone (pGHRH-R) in human papillary thyroid cancer cells:effects of GHRH antagonists on matrix metalloproteinase-2[J].Horm Cancer,2015,6(2/3):100-106.

Effect of human growth hormone releasing hormone receptor splice variant type 1 on proliferation of human liver cancer HepG2 cells

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