Abstract: Objective:To observe the effects of myricetin on the proliferation, apoptosis, migration and invasion of mouse glioma GL261 cells, and to provide theoretical basis for myricetin in treating glioma. Methods:The mouse glioma GL261 cells were cultured in vitro. The experiment was divided into control group and different concentrations (10, 20, 30, 40, 50 and 60 μmol·L^-1) of mytricetin groups. CCK-8 assay was used to detect the survival rates of the mouse glioma GL261 cells in various groups.The cell cycle of GL261 cells in various groups was detected by flow cytometry; Hoechst 33342 staining and flow cytometry were used to detect the apoptosis of Gl261 cells in various groups. The number of migrated GL261 cells and the number of GL261 cells entering the lower chamber were detected by Transwell chamber assay. The expression levels of matrix metalloproteinases(MMPs) mRNA were detected by RT-PCR method. Results:The CCK-8 assay results showed that the survival rate of GL261 cells in 20 μmol·L^-1 myricetin group was significantly decreased compared with control group (P<0.01). The flow cytometry results showed that the proportion of GL261 cells in G₁ phase in 40 μmol·L^-1 myricetin group was significantly increased (P<0.05) and the proportion of GL261 cells in S phase was decreased(P<0.05) compared with control group.The Hoechst 33342 staining results showed that the apoptotic bodies appeared in myricetin groups.The early and late apoptotic rates of GL261 cells in myricetin groups detected by AnnexinⅤ/PI double staining were increased compared with control group(P<0.05). Compared with control group,the number of migrated GL261 cells in 5,10,and 20 μmol·L^-1 myricetin groups was decreased in Traswell chamber assay (P<0.05).Compared with control group,the number of GL261 cells entering the lower chamber in myricetin groups was decreased significantly(P<0.05);the number of GL261 cells passing the Matrigel was gradully decreased with the increasing of the concentrations of myricetin.Compared with control group,the expression levels of MMP-2 and MMP-9 mRNA in the GL261 cells in 5 and 10 μmol·L^-1 myricetin groups were decreased(P<0.05). Conclusion:Myricetin could inhibit the proliferation of GL261 cells and induce the apoptosis. Meanwhile, it could also inhibit the migration and invasion of GL261 cells.

Key words: glioma, GL261 cells, myricetin, cell proliferation, cell invasion, apoptosis, matrix metalloproteinases