Journal of Jilin University Medicine Edition ›› 2018, Vol. 44 ›› Issue (01): 101-105.doi: 10.13481/j.1671-587x.20180119

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Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG2 cells

ZHAO Yanying1, WANG Xiuyan2, QIN Guotao1, SUN Yuanjie1, LIU Zhizhong1, LI Ranwei2   

  1. 1. Department of Gastroenterology, Fourth Hospital, Jilin University, Changchun 130011, China;
    2. Department of Urinary Surgery, Second Hospital, Jilin University, Changchun 130041, China
  • Received:2017-06-02 Online:2018-01-28 Published:2018-01-24

Abstract: Objective: To study the effect of vascular endothelial growth factor 165b (VEGF165b) on the biological characteristics of human hepatocellular carcinoma HepG2 cells,and to explore its mechanism. Methods: The HepG2 cells were divided into blank group (treated with transfection reagent),control group(transfected with pcDNA3.0 expression vector)and pcDNA-VEGF165b group(transfected with pcNDA-VEGF165b).MTT assay was used to detect the survival rate of HepG2 cells;RT-PCR and Western blotting method were used to detect the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG2 cells;Transwell chamber assay was used to measure the migration ability of HepG2 cells. Results: Compared with blank group,there was no significant changes in the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG2 cells in control group(P>0.05).Compared with blank group,the expression levels of VEGF165b mRNA and protein in the HepG2 cells in PcDNA-VEGF165b group were significantly increased (P<0.05),and the expression levels of VEGF165 mRNA and protein were significantly decreased(P<0.05).There was no significant difference in the survival rates between blank group and control group (P>0.05).The survival rate of HepG2 cells in PcDNA-VEGF165b group was lower than those in blank group and control group,but the differences were not statistically significant (P>0.05).The cell migration experiment results showed that there was no significant difference in the migration rate between blank group and control group (P<0.05).Compared with blank group and control group,the migration rate of HepG2 cells in PcDNA-VEGF165b group was significantly reduced(P<0.05). Conclusion: VEGF165b can inhibit the expressions of VEGF165 mRNA and protein,and VEGF165b has no effect on the proliferation of hepatocellular carcinoma cells; but it can reduce the migration of hepatocellular carcinoma cells.

Key words: liver neoplasms, vascular endothelial growth factor 165, cell migration, HepG2 cells, vascular endothelial growth factor 165b, cell proliferation

CLC Number: 

  • R735.7