多囊卵巢综合征患者与正常排卵妇女GV期卵母细胞转录组的比较

doi:10.13481/j.1671-587x.20180321

Abstract

Abstract: Objective: To investigate the effects of controlled ovarian stimulation(COS) on the differentially expressed genes in GV oocytes and main signal transduction pathways in the polycystic ovary syndrome (PCOS) patients and the normal ovulatory women,and to screen the key genes impacting the development of oocytes of the PCOS patients. Methods: During controll ovarian hyperstimulation with GnRH-a long protocol, 3 patients with PCOS(PCOS group) and 3 normal ovulatory women due to male infertility factor (control group)were selected.Enzyme digestion was used to islolate the granule cells.The dumped immature oocytes were collected after intracytoplasmic sperm injection(ICSI).The cDNA library was constructed and sequencing was performed in Illumina MiSeq sequencing platform and RT-PCR was used to confirm the data obtained in vivo. Results: A total of 510 024 82 sequence reads were obtained,and 8 G base sequence information were contained.A total of 63 differentially expressed genes were found by bio-informatics software,including 19 significant up-regulation genes and 44 significant down-regulation genes (Fold Change>4,FDR<0.01).The expression levels of vascular endothelial growth(VEGF) and fatly acid dehydrogenase 1(FADS1) mRNA in the patients in PCOS group were significantly higher than those in control groups(P<0.05),and the expression levels of Runt-related transcription factor 2(RUNX2),chemokine factor 1 (CXCL1) and heat shock protein27(Hsp27) mRNA were significantly lower than those in control group (P<0.05).These above results were consistent with the functional verification results.According to the GO function annotation,these genes were divided into several branches,including phosphorylation,autophagy,and transcriptional regulation,etc.Referring to the KEGG database,the differential genes were mainly associated with phosphatidylinositol -3-kinase (PI3K-Akt),transforming growth factor β(TGF-β) Hippo,p53 and peroxisome proliferators-activated receptor(PPAR) signal pathways. Conclusion: The differentially expressed genes in GV oocytes of the PCOS patients may affect the development of follicle and maturation of oocytes and lead to poor quality of embryo.

Key words: polycystic ovary syndrome, controlled ovarian stimulation, oocytes, high-throughput sequencing

CLC Number:

Q344.13

DU Chen, CHEN Xiujuan, HOU Shilei, ZHAO Jie. Comparsion of GV oocytes transcriptome between PCOS patients and normal ovulatory women[J].Journal of Jilin University Medicine Edition, 2018, 44(03): 568-573.

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Comparsion of GV oocytes transcriptome between PCOS patients and normal ovulatory women

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