小鼠骨髓间充质干细胞对人舌鳞状细胞癌Cal27细胞的归巢作用及其对Cal27细胞增殖和迁移的促进作用

doi:10.13481/j.1671-587x.20180501

Abstract

Abstract: Objective:To explore the homing effect of mouse bone marrow-derived mesenchymal stem cells (mBMSCs) on the tongue squamous cell carcinoma Cal27 cells, and to observe the biological behaviors of tongue squamous cell carcinoma Cal27 cells affected by mBMSCs. Methods:The mBMSCs were isolated and purified by whole bone marrow methods. The cell phenotype was analyzed by flow cytometry to identify the mBMSCs. The mBMSCs were divided into blank control group, Hacat cell control group and Cal27 cell experiment Cal27 cell group. After co-cultured with normal culture medium, Hacat conditioned medium and Cal27 conditioned medium,the number of the migrated Cal27 cells was detected by cell migration assay. The Cal27 cells were divided into single Cal27 cell control group and co-cultured Cal27 and mBMSCs experiment group(co-cultured group). The Cal27 cells in co-cultured group were co-cultured with mBMSCs using Transwell method. Cell proliferation assay and colony formation assay were used to detect the proliferation ability of the co-cultured Cal27cells. Cell migration assay was performed for the detection of the migration ability of the Cal27 cells after co-culture. Results:The inverted microscope results showed that most mBMSCs were short shuttle-like, with abundant cytoplasm, oval or nephron nucleus and irregular cell lengths; the cells arranged well. The results of flow cytometry showed that CD44, CD29, and Sca-1 were positive, and CD45, CD11b were negative in the mBMSCs. Compared with blank control group and Hacat cell control group, the number of mBMSCs homing to Cal27 cells in Cal27 cell group was significantly increased (P<0.01). Compared with single Cal27 cell control group, the growth rates of Cal27 cells in co-cultured group were significantly increased from the 3rd day after co-culture(P<0.01), and the number of clones was increased. The cell migration test results showed that the number of transmembrane cells in co-cultured group was significantly increased compared with single Cal27 cell control group (P<0.01). Conclusion:mBMSCs can home to the tongue squamous cell carcinoma Cal27 cells, which promotes the proliferation and migration of tongue squamous cell carcinoma Cal27 cells and promotes the development of tongue squamous cell carcinoma. So mBMSCs can be used as an anti-tumor target.

Key words: bone marrow-derived mesenchymal stem cells, oral squamous cell carcinoma, co-culture, homing

CLC Number:

R73-37

MENG Lin, WANG Lu, BU Wenhuan, DING Xinxin, GAO Huali, WANG Zilin, LIU Yulan, LI Chen, SUN Hongchen. Homing effect of mouse bone marrow-derived mesenchymal stem cells on human tongue squamous cell carcinoma Cal27 cells and its promotion on proliferation and migration of Cal27 cells[J].Journal of Jilin University(Medicine Edition), 2018, 44(05): 891-896.

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Homing effect of mouse bone marrow-derived mesenchymal stem cells on human tongue squamous cell carcinoma Cal27 cells and its promotion on proliferation and migration of Cal27 cells

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