Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (05): 892-896.doi: 10.13481/j.1671-587x.20160510

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Inhibitory effect of poly(lactic acid) electrospun membranes loaded with cisplatin and chloroquine on proliferation of oral squamous cell carcinoma CAL-27 cells

ZHOU Lijia1,2, XU Zhaonan1, BI Ye1, YANG He1, ZHANG Zebing1, WANG Shuyu1, JIA Jie3   

  1. 1. Department of Pathology, Hospital of Stomatology, Jilin university, Changchun 130021, China;
    2. Department of Clinical Medicine, Daqing Medcial College, Daqing 163312, China;
    3. Department of Eelectronic Diagnosis, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2016-01-24 Online:2016-09-28 Published:2016-09-29

Abstract:

Objective: To investigate the effect of poly(lactic acid) (PLA) electrospun membranes loaded with cisplatin and chloroquine on the oral squamous cell carcinoma CAL-27 cells,and to explore the method to prevent the recurrence of oral cancer. Methods: The DDP/PLA membranes,CQ/DDP/CQ/PLA membranes and CQ/DDP/PLA membranes were prepared by electrospinning. Then the micro morphology of three kinds of membranes were observed by scanning electron microscope(SEM);the degradation rate of PLA membrane was measuredby UV spectrophotometric. The LC3-Ⅱ expression level in CAL-27 cells was detected by laser scanning confocal microscope.The survival rate of CAL-27 cells was detected by MTT method. Results: The SEM results showed that the nanofibers of DDP/PLA,CQ/DDP/PLA and CQ/DDP/CQ/PLA membranes were continuous and smooth with uniform diameters. The degrated time of membranes was about 21 d. The MTT result showed that compared with control group,at first,the effects of cell killing of DDP/PLA membranes, CQ/DDP/CQ/PLA membranes and CQ/DDP/PLA membranes were not obvious; as the extension of time, the survival rates of CAL-27 cells in DDP/PLA membranes group,CQ/DDP/CQ/PLA membranes group and CQ/DDP/PLA membranes group were decreased(P<0.05). The immunofluorescence results showed that the fluorescence intensity of LC3-Ⅱ in CQ/DDP/CQ/PLA membranes group and CQ/DDP/PLA membranes group were lower than that in DDP/PLA membranes group. Conclusion: CQ/DDP/PLA membranes with sustained-release effect can increase the sensitivity of CAL-27 cells to DDP and enchance the killer effect of DDP on the CAL-27 cells.

Key words: electrospinning, autophagy, cisplatin, chloroquine, oral squamous cell carcinoma

CLC Number: 

  • R780.2