形觉剥夺性近视模型大鼠巩膜成纤维细胞中TGF-β1表达及Wnt/β-catenin信号通路的调控作用

doi:10.13481/j.1671-587x.20190420

Abstract

Abstract: Objective:To construct the form deprivation myopia (FDM) rat models, and to elucidate the expression of transforming growth factor-β1 (TGF-β1) in scleral fibroblasts of the FDM rats and its relationship with Wnt/β-catenin signaling pathway. Methods:Forty rats were randomly divided into control group and FDM model group, with 20 rats in each group.The FDM rat model was established in FDM model group.The axial lengths of the rats were determined.The rat sclera tissue of eyeball was separated.The expression levels of TGF-β1 protein and mRNA in sclera tissue of the rats were determined by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) methods.The scleral fibroblasts of rats were isolated and cultured.The fibroblasts in the sclera of the rats in control group were used as the control group.The fibroblasts in FDM model group were divided into FDM group and FDM+Dickkopf related protein 1 (DDK1) group (added to DDK1 to culture).The expression levels of TGF-β1, Dicer-like 3 (DCL3), colon adenomatous polyp protein (APC), glycogen synthase kinase 3β (GSK3β), p21-GSK3β, and β-catenin protein and mRNA in scleral fibroblasts were determined by Western blotting and RT-PCR methods. Results:Compared with control group,the axial length of the rats in FDM group was increased (P<0.01);the expression levels of TGF-β1 protein and mRNA in sclera tissue were significantly decreased(P<0.01).There were no significant differences in the expression levels of GSK3β protein and mRNA in scleral fibroblasts between control group,FDM group and FDM+DDK1 group (P>0.05);but there were significant differences in the expression levels of TGF-β1,DCL3,APC,p21-GSK3β,and β-catenin protein and mRNA in the scleral fibroblasts(P<0.01).Compared with control group, the expression levels of TGF-β1 and APC protein and mRNA in scleral fibroblasts of the rats in FDM group were decreased(P<0.01), and the expression levels of DCL3, p21-GSK3β, and β-catenin protein and mRNA were increased(P<0.05).Compared with FDM group, the expression levels of TGF-β1 and APC protein and mRNA in the scleral fibroblasts of the rats in FDM+DDK1 group were increased(P<0.01), and the expression levels of DCL3, p21-GSK3β, and β-catenin protein and mRNA were decreased(P<0.01). Conclusion:The expression level of TGF-β1 in the scleral fibroblasts of the FDM model rats is decreased, and its level is regulated by the Wnt/β-catenin signaling pathway.

Key words: myopia, sclera, fibroblasts, transforming growth factor-β1, Wnt/β catenin, rats,SD

CLC Number:

R778.11

ZHANG Xin, JU Zhaojuan, ZHANG Jian, ZHAO Yan. Expression of TGF-β1 in scleral fibroblasts of rats with form deprivation myopia and regulation of Wnt/β-catenin signaling pathway[J].Journal of Jilin University(Medicine Edition), 2019, 45(04): 861-866.

References

[1] XIONG SY, SANKARIDURG P, NADUVILATH T,et al.Time spent in outdoor activities in relation tomyopiaprevention and control:A meta-analysis and systematic review[J].Acta Ophthalmol,2017,95(6):551-566.
[2] SUHR THYKJAER A, LUNDBERG K, GRAUSLUND J.Physical activity in relation to development and progression of myopia-a systematic review[J].Acta Ophthalmol,2017,95(7):651-659.
[3] SEKO Y, AZUMA N, YOKOI T,et al.Anteroposterior patterning of gene expression in the human infant sclera:chondrogenic potential and wnt signaling[J].Curr Eye Res,2017,42(1):145-154.
[4] ZHANG Y, ZHAO D W, YANG S,et al.Protective effects of fucoidan on epithelial-mesenchymal transition of retinal pigment epithelial cells and progression of proliferative vitreoretinopathy[J].Cell Physiol Biochem,2018,46(4):1704-1715.
[5] 左韬,马贤德,张祝强,等.形觉剥夺性近视模型大鼠巩膜组织中bFGF、TGF-β mRNA及蛋白表达规律的实验研究[J].辽宁中医杂志,2014,41(9):2001-2004.
[6] GALVIS V, TELLO A, CAMACHO P A,et al.Bio-environmental factors associated withmyopia:An updated review[J].Arch Soc Esp Oftalmol,2017,92(7):307-325.
[7] 石晶,谭小波,杨洁,等.虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响[J].吉林大学学报:医学版,2017,43(5):932-936.
[8] SANKARIDURG P.Contact lenses to slow progression of myopia[J].Clin Exp Optom,2017,100(5):432-437.
[9] IRVING E L, YAKOBCHUK-STANGER C.Myopia progression control lens reverses induced myopia in chicks[J].Ophthalmic Physiol Opt,2017,37(5):576-584.
[10] PARK G B, KIM D.Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway[J].Mol Med Rep,2018,17(3):3563-3574.
[11] IRNATEN M, ZHDANOV A, BRENNAN D,et al.Activation of the NFAT-calcium signaling pathway in human lamina cribrosa cells in glaucoma[J].Invest Ophthalmol Vis Sci,2018,59(2):831-842.
[12] 张兰兰,刘琼,于健,等.转化生长因子-β(TGF-β)影响豚鼠巩膜成纤维细胞增殖和α-平滑肌肌动蛋白(α-SMA)表达的研究[J].眼科新进展,2016,36(11):1011-1015.
[13] WEI Q, LIU Q, REN C,et al.Effects of bradykinin on TGF β1 induced epithelial mesenchymal transition in ARPE 19 cells[J].Mol Med Rep,2018,17(4):5878-5886.
[14] VALLÉE A, LECARPENTIER Y, GUILLEVIN R,et al.Interactions between TGF-β1, canonical WNT/β-catenin pathway and PPAR γ in radiation-induced fibrosis[J].Oncotarget,2017,8(52):90579-90604.
[15] MAZZONI J, SMITH J R, SHAHRIAR S,et al.The Wnt inhibitor apcdd1 coordinates Vascular remodeling and barrier maturation of retinal blood vessels[J].Neuron,2017,96(5):1055-1069.e6.
[16] ZHANG B X, SHEN C F, GE F Y,et al.Epigenetically controlled Six3 expression regulates glioblastoma cell proliferation and invasion alongside modulating the activation levels of WNT pathway members[J].J Neurooncol,2017,133(3):509-518.
[17] GONG Y Y, PENG M Y, YIN D Q,et al.Long non-coding RNA H19 promotes the osteogenic differentiation of rat ectomesenchymal stem cells via Wnt/β-catenin signaling pathway[J].Eur Rev Med Pharmacol Sci,2018,22(24):8805-8813.
[18] MA S, LEI Y, ZHANG L,et al.Research on the inhibiting effect of tanshinone ⅡA on colon cancer cell growth via COX-2-Wnt/β-catenin signaling pathway[J].J BUON,2018,23(5):1337-1342.
[19] LIU M, GAO X, LIU C L.Increased expression of lncRNA FTH1P3 promotes oral squamous cell carcinoma cells migration and invasion by enhancing PI3K/Akt/GSK3b/Wnt/β-catenin signaling[J].Eur Rev Med Pharmacol Sci,2018,22(23):8306-8314.
[20] GAO L, XU F M, SHI W J,High-glucose promotes proliferation of human bladder cancer T24 cells by activating Wnt/β-catenin signaling pathway[J].Eur Rev Med Pharmacol Sci,2018,22(23):8151-8160.
[21] MA M M, ZHANG Z W, DU E G,et al.Wnt signaling in form deprivation myopia of the mice retina[J].PLoS One,2014,9(4):e91086.

Expression of TGF-β1 in scleral fibroblasts of rats with form deprivation myopia and regulation of Wnt/β-catenin signaling pathway

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHOU Licheng, LIU Xianfa, LI Qiang, LI Rong, HUANG Jiagan, ZHANG Qiong, LI Xiaofei. Anti-atherosclerosis effect of berberine combined with simvastatin in atherosclerosis model rats and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 849-854.
[2]	LIU Jingqiao, ZHENG Yan, WANG Yujing, MENG Yali, XU Shuwen, ZHANG Hualin. Improvement effect of estradiol valerate combined with aspirin on intrauterine adhesion in rats [J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 830-835.
[3]	WANG Qi, DU Shuai, ZHAO Quanmin, LI Qingjie. Effects of pilose antler polypeptide on abilities of proliferation and collagen secretion of mouse embryonic fibroblasts NIH/3T3 and their mechanisms [J]. Journal of Jilin University(Medicine Edition), 2019, 45(02): 307-312.
[4]	LI Hongxia, GONG Haiye, ZENG Xiao, ZHANG Susu, LIU Bofeng. Expressions of TGF-β1 and PLGF in tissue of patients with different types of gestational trophoblastic diseases and their significances [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 1041-1046.
[5]	ZHANG Xiaohuan, DUAN Minghua, YAN Yuli, PAN Xin, LI Haiqing, ZHOU Changlong, ZHAO Tanyi. Isolation and identification of human amnion fibroblasts [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 845-848.
[6]	YAN Hui, LOU Shilei, MIAO Yongdi, QI Rui, GUO Zishuo, SUN Cong. Isolation,culture and identification of primary fibroblasts from rat colon [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 849-852.
[7]	SHI Jing, TAN Xiaobo, YANG Jie, HAO Jiaying, YANG Weili, LI Na. Influence of astaxanthin in refractive status and pathohistology of myopia models caused by concave lens of guinea pigs [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 932-936.
[8]	WANG Liping, WANG Jianhui, LI Shumin, YANG Xiuhong. Effect of N-acetyl cysteine pathway on expression of intermediate conductance Ca²⁺-activated K⁺ channels in cardiac fibroblasts of AGT-REN double transgenic hypertension mice [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 881-886.
[9]	REN Liwei, WEI Pei, YANG Xiaofei, YANG Lu, DENG Chunyan, QI Hui, LI Furong. Construction of induced mouse pluripotent stem cells by piggyBac transposon and its identification [J]. Journal of Jilin University Medicine Edition, 2016, 42(04): 676-680.
[10]	AN Ying, LU Qian, XU Congfei, WANG Yanchun, HAN Liqin. Inhibitory effect of Rhodiola total flavonoids on proliferation of cardiac fibroblasts in rats [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1190-1194.
[11]	LIU Yan, GUO Jun, QI Changhai, GUO Jinghui, CHENG Yun, LIANG Lei. Analysis on correlation between quantitative results and semi-quantitative scores from ultrasound elastography and distribution of myofibroblasts in breast tumor [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1288-1292.
[12]	ZHANG Xiao, WANG Ping, YU Shuyuan, WANG Hongyan, TANG Yong, ZHOU Yulai, ZHU Wei. Changes of proliferation and phenotypes of epithelial cells and fibroblasts co-cultured on SF/PLLA composite fibrous scaffolds [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 774-778.
[13]	WANG Hongyue, WANG Mo, ZHANG Chen, QI Hongxin, CHI Baorong. Effects of hepatocyte growth factor and angiotensin Ⅱ on transdifferentiation of tubular epithelial-myofibroblasts and their mechanisms [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 798-801.
[14]	. Influence of tripterygium glycosides in BMP-2 expression in fibroblasts of patients with ankylosing spondylitis [J]. Journal of Jilin University Medicine Edition, 2014, 40(06): 1187-1191.
[15]	LIU Shan-shan,GAO Han-nan,ZHANG Lian-bo,SONG Wen-gang,SUN Ying,SUN Shi-lon. Effect of ionizing radiation on expression levels of hyaluronic acid protein and \|HAase1 mRNA in human fibroblasts [J]. J4, 2012, 38(3): 393-398.