神经生长因子对比格犬年轻恒牙牙髓组织再生的促进作用

doi:10.13481/j.1671-587x.20190518

Abstract

Abstract: Objective:To establish models of young periapical periodontitis in the Beagle dogs and to explore the effects of nerve growth factor (NGF) on the regeneration of young permanent teeth pulp tissue of the Beagle dogs, and clarify the action mechanism. Methods:Twelve permanent teeth of 6 Beagle dogs were selected and divided into control group, hydrogel group, and hydrogel combined with NGF group, with 4 permanent dental of 2 Beagle dogs in each group.The teeth in control group were not treated.The teeth in hydrogel group were established the periapical periodontitis models and were treated with hydrogel alone.The teeth in hydrogel combined with NGF group were established the models of periapical periodontitis and treated with NGF combined with hydrogel.The histological changes of the experimental teeth in various groups were observed by HE staining.The depth of regenerated tissue and the area of regenerated tissue of the experimental teeth in various groups were measured. Results:The young permanent periapical periodontitis models of Beagle dogs were successfully established.In control group,the dental pulp tissue in the root canal was normal, and it was composed of fibroblasts, odontoblasts and blood vessels;in hydrogel group,there was no new hard tissue formed at the apex, and fiber repair occured at the apical period;in hydrogel combined with NGF group, there was newly formed hard tissue in the root canal,the cementoblast-like cells and the cementum matrix lacuna could be seen,the free cementum-like tissue was seen in the root canal,and the newly formed soft tissue in the root canal was mainly the blood vessels and the fibroblasts. The depth of regeneranted tissue and the area of regenerated tissue in hydrogel combined with NGF group were higher than those in hydrogel group (t=66.261,P<0.01;t=60.363,P<0.01). Conclusion:NGF has the effect of promoting the regeneration of dental pulp tissue of young permanent periapical periodontitis of the Beagle dogs.

Key words: nerve growth factor, periapical periodontitis, dental pulp, permanent teeth, hydrogel

CLC Number:

R781

LI Wenjing, TIAN Songbo, CHENG Dongmei, LIU Congna, CUI Zhanqin. Promotion effect of nerve growth factor on regeneration of young permanent dental pulp tissue in Beagle dogs[J].Journal of Jilin University(Medicine Edition), 2019, 45(05): 1075-1079.

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Promotion effect of nerve growth factor on regeneration of young permanent dental pulp tissue in Beagle dogs

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