不同周龄小鼠耳蜗组织中Otos的表达及其意义

doi:10.13481/j.1671-587x.20190618

Abstract

Abstract: Objective: To observe the expressions and distribution of Otos in cochlea tissue of the mice with age-related hearing loss (AHL) at different ages,and to explore its relationship with AHL. Methods: A total of 40 SPF C57BL/6Cnc mice were divided into 4-week-old group,16-week-old group,32-week-old group and 48-week-old group,and there were 10 mice in each group. The auditory brainstem response (ABR) was performed in the mice in various groups,then after the cochlea was removed,the expressions and distribution of Otospiralin protein in cochlea tissue of the mice in various groups were detected by immunofluorescence;the expression levels of Otos mRNA in cochlea tissue of the mice in various groups were examined by qRT-PCR method,and the expression levels of Otospiralin protein in cochlea tissue of the mice in various groups were detected by Western blotting method. Results: The Otospiralin proein was mainly expressed in the spiral ligament of the cochlea tissue of the mice and was expressed in all five fibrocytes;compared with 4-week-old group,the expression amounts of Otospiralin protein in cochlea tissue of the mice in 16-,32-,and 48-week-old groups were significantly increased(P<0.01).Compared with 4-week-old group,the mean ABR thresholds of the mice in 16-,32- and 48-week-old groups were significantly increased (P<0.01),and the expression levels of Otos mRNA and Otospiralin protein in cochlea tissue of the mice were significantly decreased (P<0.01);compared with 16-week-old group,the mean ABR thresholds of the mice in 32- and 48-week-old groups were significantly increased (P<0.01),and the expression levels of Otos mRNA and Otospiralin protein in cochlea tissue of the mice were significantly decreased (P<0.01); compared with 32-week-old group,the mean ABR threshold of the mice in 48-week-old group was significantly increased (P<0.01),and the expression levels of Otos mRNA and Otospiralin protein in cochlea tissue of the mice were significantly decreased (P<0.01). Conclusion: With the increasing of age,the auditory function of AHL mice is declined gradually,and the expression levels of Otos mRNA and Otospiralin protein are decreased gradually.The changes of Otos may be involved in the occurrence and development of AHL.

Key words: auditory brainstem response, Otos, age-related hearing loss, presbycusis, spiral ligament

CLC Number:

R764.43

SHI Sunan, LIU Hao, GONG Liang. Expressions of Otos in cochlear tissue of mice at different ages and their significances[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1299-1304.

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Expressions of Otos in cochlear tissue of mice at different ages and their significances

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