不同时间窗高压氧干预对大鼠脊髓损伤后细胞凋亡及脊髓功能恢复的影响

doi:10.13481/j.1671-587x.20150328

Abstract

Abstract:

Objective To study the effects of hyperbaric oxygen at different intervention time on the apoptosis and functional recovery following spinal cord injury(SCI) in the rats and to investigate the ideal application method of hyperbaric oxygen, and to provide some experimental basis for clinical hyperbaric oxygen therapy for SCI. Methods 160 SD rats were randomly divided into normal injury group, hyperbaric oxygen preconditioning(HBO-PC) group, hyperbaric oxygen therapy(HBOT)group and HBO-PC+HBOT group.The rat models of SCI were established with Allen's dropping weight technique.The rats in normal injury group weren't treated with any intervention;the rats in HBO-PC group were treated with hyperbaric oxygen intervention 10 d before modeling;the rats in HBOT group were treated with hyperbaric oxygen intervention only after modeling;the rats HBO-PC+HBOT group were treated with hyperbaric oxygen intervention before and after modeling.The segments of injured spinal cord were collected from the rats in 4 groups on the 1st, 2nd, 7th and 14th day after injury.The expression levels of Fas and FasL were detected with immunnohistochemistry PV-9000 two steps method.The apoptotic index(AI) was detected with TUNEL technique.The neurological function of spinal cord was assessed by BBB score. Results Compared with normal injury group, the expression levels of Fas and FasL of the rats in HBO-PC group, HBOT group and HBO-PC+HBOTgroup were decreased significantly(P<0.01);there were significant differences between HBO-PC+HBOT group and HBO-PC group, HBOT group(P<0.05), and there was no significant difference between HBO-PC group and HBOT group(P>0.05).Compared with normal injury group, the AI of the cells in HBO-PC group, HBOT group and HBO-PC+HBOT group were decreased significantly(P<0.01);compared with HBO-PC group and HBOT group, the AI of the cells in HBO-PC+HBOT group was decreased significantly(P<0.05);there was no significant difference between HBO-PC group and HBOT group(P>0.05).Compared with normal injury group, the BBB scores of the rats in HBO-PC group, HBOT group and HBO-PC+HBOT group 7 and 14 d after operation were increased significantly(P<0.01);compared with HBO-PC group and HBOT group, the BBB scores in HBO-PC+HBOT group were increased significantly (P<0.05 or P<0.01);compared with HBO-PC group, the BBB scores in HBOT group were increased significantly (P<0.05). Conclusion Hyperbaric oxygen can inhibit the apoptosis after SCI, which contributes to the recovery of locomotor performances in the rats.The best intervention time is in front of the damage.The method of HBO-PC combined with HBOT is the best way for the treatment of SCI.

Key words: spinal cord injury, hyperbaric oxygen, intervention time, Fas, FasL, apoptotosis

CLC Number:

R651.2

LIU Fang, CHEN Jia, SU Hua. Effects of hyperbaric oxygen at different intervention time on apoptosis and functional recovery following spinal cord injury in rats[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 578-583.

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Effects of hyperbaric oxygen at different intervention time on apoptosis and functional recovery following spinal cord injury in rats

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