YAP对非小细胞肺癌细胞增殖和迁移能力的影响

doi:10.13481/j.1671-587x.20190622

Abstract

Abstract: Objective: To investigate the role of long non-coding RNA(lncRNA) MALAT1 in the proliferation and migration of YAP-mediated non-small cell lung cancer (NSCLC) cells,and to elucidate its mechanism. Methods: The A549 and H1299 cell lines were selected and divided into Scramble siRNA group(transfected with Scramble siRNA),siYAP-1 group(transfected with siYAP-1)and siYAP-2 group(transfected with siYAP-2). RT-PCR and Western blotting methods were used to detect the expression levels of YAP and MALAT1 mRNA and YAP protein in the cells in variouis groups. The pcDNA3.1-YAP plasmid(pcDNA3.1-YAP group), pcDNA3.1-YAP plasmid plus siMALAT1-2 (pcDNA3.1-YAP+siMALAT1-2 group)and the control plasmid (pcDNA3.1-YAP+Scramble siRNA group)were transfected into the A549 and H1299 cells, respectively.CCK-8 method and scratch assay were used to detect the proliferation and migration abilities of the cells in various groups. Results: The expression levels of YAP mRNA and protein in the A549 and H1299 cells in siYAP-1 and siYAP-2 groups were significantly lower than those in Scramble siRNA group(P<0.05), and the expression levels of MALAT1 mRNA in the cells in siYAP-1 and siYAP-2 groups were significantly lower than those in Scramble siRNA group (P<0.05).Compared with pcDNA3.1-YAP group, the proliferation abilities of the A549 and H1299 cells in pcDNA3.1-YAP+siMALAT1-2 group were decreased(P<0.05),the migration ability of the H1299 cells in pcDNA3.1-YAP+siMALAT1-2 group was decreased(P<0.05). Conclusion: In the NSCLC cells, YAP promotes the cell proliferation and migration by regulating the expression of lncRNA MALAT1.

Key words: YAP, MALAT1, lung neoplasms, cell proliferation, migration

CLC Number:

R734.2

ZHOU Ning, GUO Jiwei, DAI Juanjuan, LI Xuelin, XI Sichuan, GONG Kaikai, WU Yan. Effect of YAP on proliferation and migration of non-small cell lung cancer cells[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1320-1326.

References

[1] HERBST R S, MORGENSZTERN D, BOSHOFF C. The biology and management of non-small cell lung cancer[J]. Nature, 2018,553(7689):446-454.
[2] WEI T, WEILER S, TÓTH M, et al. YAP-dependent induction of UHMK1 supports nuclear enrichment of the oncogene MYBL2 and proliferation in liver cancer cells[J]. Oncogene, 2019, 38(27):5541-5550.
[3] ZHAO B, LI L, LEI Q Y, et al. The Hippo-YAP pathway in organ size control and tumorigenesis: An updated version[J]. Genes Dev, 2010,24(9):862-874.
[4] HAO Y W, CHUN A, CHEUNG K, et al. Tumor suppressor LATS1 is a negative regulator of oncogene YAP[J]. J Biol Chem, 2008,283(9):5496-5509.
[5] LAI C J, LIN C Y, LIAO W Y, et al. CD44 promotes migration and invasion of docetaxel-resistant prostate cancer cells likely via induction of hippo-Yap signaling[J]. Cells, 2019,8(4):E295.
[6] MOROOSHI T, HAYASHI T, PAN W W, et al. The hippo pathway kinases LATS1/2 suppress cancer immunity[J]. Cell, 2016,167(6):1525-1539.
[7] LO S F, STRANO S, BLANDINO G. YAP and TAZ in lung cancer: oncogenic role and clinical targeting[J]. Cancers (Basel), 2018,10(5):E137.
[8] WANG E Y, CHENG J C, THAKUR A, et al. YAP transcriptionally regulates ErbB2 to promote liver cell proliferation[J]. Biochim Biophys Acta, 2018,1861(9):854-863.
[9] ZHUANG M, ZHAO S L, JIANG Z, et al. MALAT1 sponges miR-106b-5p to promote the invasion and metastasis of colorectal cancer via SLAIN2 enhanced microtubules mobility[J]. EBioMedicine, 2019,41:286-298.
[10] MOROISHI T, HAYASHI T, Pan W W, et al. The hippo pathway kinases lats1/2 suppress cancer immunity[J]. Cell, 2016,167(6):1525-1539,e17.
[11] ZHAO Z, CHEN C, LIU Y, et al. 17β-Estradiol treatment inhibits breast cell proliferation, migration and invasion by decreasing MALAT-1 RNA level[J]. Biochem Biophys Res Commun, 2014,445(2):388-393.
[12] WANG J Y, WANG H M, ZHANG Y, et al. Mutual inhibition between YAP and SRSF1 maintains long non-coding RNA, Malat1-induced tumourigenesis in liver cancer[J]. Cell Signal, 2014,26(5):1048-1059.
[13] CHAN S W, LIM C J, CHONG Y F, et al. Hippo pathway-independent restriction of TAZ and YAP by angiomotin[J]. J Biol Chem, 2011,286(9):7018-7026.
[14] LIANG Z G, WANG Y Y, SHEN Z Y, et al. Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling[J]. Tumour Biol, 2016,37(8):10909-10915.
[15] GUO J W, WU Y, YANG L J, et al. Repression of YAP by NCTD disrupts NSCLC progression[J]. Oncotarget, 2017,8(2):2307-2319.
[16] TRIPATHI V, ELLIS J D, SHEN Z, et al. The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation[J]. Mol Cell, 2010,39(6):925-938.
[17] MACIAS S, PLASS M, STAJUDA A, et al. DGCR8 HITS-CLIP reveals novel functions for the microprocessor[J]. Nat Struct Mol Biol, 2012,19(8): 760-766.
[18] MIYAGAWA R, TANO K, MIZUNO R, et al. Identification of cis-and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles[J]. RNA, 2012,18(4):738-751.
[19] JI P, DIEDERICHS S, WANG W B, et al. MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer[J]. Oncogene, 2003,22(39):8031-8041.
[20] GUTSCHER T, HÄMMERLE M, EISSMANN M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells[J]. Cancer Res, 2013,73(3):1180-1189.
[21] TANO K, MIZUNO R, OKADA T, et al. MALAT-1 enhances cell motility of lung adenocarcinoma cells by influencing the expression of motility-related genes[J]. FEBS Lett, 2010,584(22):4575-4580.
[22] ZHAO J, LI X N, YANG Y, et al. Effect of YAP1 silencing on esophageal cancer[J]. Onco Targets Ther, 2016,9:3137-3146.
[23] DONG L, LIN F, WU W J, et al. Transcriptional cofactor Mask2 is required for YAP-induced cell growth and migration in bladder cancer cell[J]. J Cancer, 2016,7(14):2132-2138.
[24] ZANCONATO F, CORDENONSI M, PICCOLO S. YAP/TAZ at the roots of cancer[J]. Cancer Cell, 2016,29(6):783-803.

Effect of YAP on proliferation and migration of non-small cell lung cancer cells

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	MENG Xuya, LIU Jie, WANG Lu, BU Wenhuan, LU Jinjin, SUN Hongchen. Effects of ascorbic acid-polyethyleneimine carbon dots on proliferation, apoptosis and oxidative stress of MG63 cells by Golgi stress [J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1218-1223.
[2]	YANG Zhiyuan, WEN Naiyan, LIN Yang, LIANG Hang, WANG Qian, HU Xindan, ZHANG Ling, REN Hui, GUO Baofeng. Inhibitory effect of SF2523 on proliferation of human glioma stem cells TS576 and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1281-1287.
[3]	LIU Yi, ZHU Lin, KANG Min. Effect of miR-302b-3p on proliferation and apoptosis of esophageal cancer EC-109 cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1346-1352.
[4]	ZHOU Chun, WANG Guanghua, ZHANG Bangzhu. Effects of miR-367 on proliferation, invasion and migration of human breast cancer MCF-7 cells and their mechanisms [J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1353-1360.
[5]	YANG Zhuangqing, LU Mei, YANG Xiaojuan, WANG Chang'an, ZOU Jieya. Inhibitory effect of inhibiting TRIM24 gene expression on proliferation, apopotosis,invasion and migration of breast cancer MCF-7 cells [J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1379-1383.
[6]	LI Menghong, JIANG Huan, WANG Liuyi, FENG Xu, LIU Nan, HU Min. Promotion effect of ursolic acid on differentiation of mouse cementoblast cell line OCCM-30 cells [J]. Journal of Jilin University(Medicine Edition), 2019, 45(05): 986-991.
[7]	ZHAO Liyan, SONG Yang, CHEN Yong, JIA Mingbo, LI Yunqian. Effect of silencing ZEB1 gene on epithelial to mesenchymal transition in glioma U87 cells [J]. Journal of Jilin University(Medicine Edition), 2019, 45(05): 1036-1040.
[8]	LIU Fenxia, CHEN Li, SUN Ning, WU Yawei. Effect of Helicobacter pylori virulence factor CagA-mediated activation of ERK signaling pathway on proliferation and apoptosis of gastric cancer cells [J]. Journal of Jilin University(Medicine Edition), 2019, 45(05): 1063-1068.
[9]	HUO Xiaolei, PEI Zhen, YANG Hao, ZHANG Yiqiang, JIA Jiantao, HAN Lingna. Effect of LncRNA-BLACAT1 on cell proliferation of non-small cell lung cancer through regulation of CyclinD1/CDKN2B axis and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 759-765.
[10]	DAI Juanjuan, YANG Lijuan, DU Jing, MIAO Shuang, XI Sichuan, LI Chen, WU Yan. Construction of PC9 cell lines stably transfectedwith human LncRNAMIR31HG gene and its effects on cell proliferation and migration [J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 801-806.
[11]	ZHANG Jie, ZHOU Hui, LIU Liang, GUO Guixi, YU Qiang, YIN Pengfei, CHEN Wenqiang, SU Xing. Effect of knockdown of EphB1 gene on oxidative damage of cardiomyocytes induced by hydrogen peroxide in rats [J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 819-824.
[12]	CHEN Xi, BAI Lin, WANG Yingying, CAO Shuang, MU Hongping, WANG Yilin, SHI Hao, ZHANG Qian, GAO Xin, ZHANG Chengyi, ZHANG Ruowen. Inhibitory effect of tanshinone ⅡA on proliferation and migration of human liver cancer HepG2 cells and its apoptosis-promoting effect [J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 531-538.
[13]	WANG Shangning, WANG Sisi, TIAN Shuyan, LI Jialin, LIU Wei. Early changes of peripheral blood lymphocyte subsets in patients with lung adenocarcinoma after single-port thoracoscopic lobectomy and their clinical significances [J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 606-613.
[14]	XU Zhenhua, SU Rongjian. Inhibitory effects of zingerone combined with sorafenib on migration and invasion of human liver cancer HepG2 cells [J]. Journal of Jilin University(Medicine Edition), 2019, 45(02): 228-233.
[15]	WANG Liuyi, LIU Nan, FENG Xu, LI Menghong, BAO Xingfu, HU Min. Effects of endothelin B receptor agonist on biological behaviors of cementoblastsof mice [J]. Journal of Jilin University(Medicine Edition), 2019, 45(02): 239-243.