Abstract: Objective: To investigate the effect of inhibiting TRIM24 gene expression on the proliferation, apoptosis, invasion and migration of breast cancer MCF-7 cells, and to elucidate the role of TRIM24 gene in the occurrence and development of breast cancer. Methods: The TRIM24 siRNA and negative control NC-siRNA were transfected into the MCF-7 cells by liposome method, and used as si-TRIM24 group and NC-siRNA group, respectively. The untransfected MCF-7 cells were used as control group. qRT-PCR and Western blotting methods were used to detect the expression levels of TRIM24 mRNA and protein in the MCF-7 cells in various groups after transfected for 48 h,MTT assay was performed to detect the proliferation activities of the MCF-7 cells in various groups,flow cytometry was used to determine the apoptotic rates of the MCF-7 cells in various groups,and Transwell assay was used to measure the number of invasion and migration cells in various groups. Results: Compared with control group and NC-siRNA group, the expression levels of and protein in the MCF-7 cells were significantly decreased (P<0.05), and the proliferation activity of the MCF-7 cells was decreased (P<0.05), the apoptotic rate of the MCF-7 cells was significantly increased (P<0.05), and the number of invasion and migration MCF-7 cells was significantly decreased (P<0.05). Conclusion: Inhibition of the expression of TRIM24 gene can significantly inhibit the proliferation, invasion and migration of breast cancer MCF-7 cells, and induce the apoptosis.

Key words: TRIM24 gene, breast neoplasms, cell proliferation, apoptosis, invasion, migration