不同强度减重步行训练对脊髓损伤模型大鼠脊髓组织中TrkB和BDNF蛋白表达水平的影响及其对运动功能恢复的促进作用

doi:10.13481/j.1671-587x.20190633

Abstract

Abstract: Objective: To observe the effects of body weight support treadmill training(BWSTT) with different exercise intensities on the expression levels of tyrosine protein kinase B (TrkB) and brains-derived neurotrophic factor (BDNF) proteins in spinal cord tissue and motor function of the spinal cord injury(SCI) rats, and to explore the possible mechanism. Methods: Fifty adult male SD rats were randomly divided into sham operation group, SCI group, BWSTT-A group(given low intersity exercise), BWSTT-B group (given middle intensity exercise)and BWSTT-C group (given high intersity exercise); there were ten rats in each group. the SCI model was prepared by using a self-made striker.The rats in sham operation group only underwent laminectomy to expose the dura mater and were sutured directly, and the rats didn't receive any treatment after surgery;the rats in SCI group weren't given any treatment after modeling;the rats in BWSTT groups were given rehabilitation exercise after SCI and the walking speeds of the rats in three groups were 7, 15, and 21 cm·s^-1, respectively.The recovery status of hind limb function of the rats in various groups was evaluated by Basso,Beattie,and Bresnahan(BBB) score 35 d after modeling;the expression levels of TrkB and BDNF proteins in spinal cord tissue of the rats in various groups were detected by Western blotting method,and the morphology and the number of Nissl bodies of the rats in various groups were analyzed by Nissl staining. Results: Compared with SCI group, the BBB scores of the rats in BWSTT-B and BWSTT-C groups were increased (P<0.01); compared with BWSTT-A group, the BBB scores of the rats in BWSTT-B and BWSTT-C groups were increased (P<0.01).Compared with sham operation group,the expression level of TrkB protein in spinal cord tissue of the rats in SCI group was decreased(P<0.01);compared with SCI group, the expression levels of TrkB protein in spinal cord tissue of the rats in BWSTT-B and BWSTT-C groups were increased (P<0.01). Compared with sham operation group, the expression level of BDNF protein in spinal cord tissue of the rats in SCI group were decreased(P<0.01); compared with SCI group, the expression levels of BDNF protein in spinal cord tissue of the rats in BWSTT-B group and BWSTT-C group were increased (P<0.01). The Nissl staining results showed that compared with SCI group, the number of Nissl cells in BWSTT groups was more and the morphology of cells was better. Conclusion: Middle and high intensities of BWSTT may promote the recovery of motor function of the SCI rats, and its mechanism may be related to up-regulation of the expressions of TrkB and BDNF proteins in spinal cord tissue.

Key words: spinal cord injury, body weight support treadmill training, motor function, brain-derived neurotrophic factor, tyrosine protein kinase B

CLC Number:

R493

AN Libin, LIU Changhong, ZHAO Bolun, ZHOU Xiaohua, LI Wentao. Effects of body weight support treadmill training with different exercise intensities on expressions of TrkB and BDNF proteins in spinal cord tissue and its promotion effect on motor function recovery of rats with spinal cord injury[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1389-1394.

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Effects of body weight support treadmill training with different exercise intensities on expressions of TrkB and BDNF proteins in spinal cord tissue and its promotion effect on motor function recovery of rats with spinal cord injury

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