Matrilin-4对大鼠牙髓损伤后修复性牙本质形成的促进作用

doi:10.13481/j.1671-587x.20200113

Abstract

Abstract: Objective: To study the effect of Matrilin-4 on the reparative dentin formation in the rats after dental pulp injury, and to explore its possibility to be used as a new capping agent. Methods: A total of 28 male Wistar rats were selected. A cavity on the occlusal surface of maxillary first molars on both sides was prepared in each rat. Matrilin-4 was applied on the left exposed pulp(Matrilin-4 group), and PBS was applied on the right exposed pulp(PBS group);and the cavities in both sides were covered with glass ions. On days 3, 7, 14 and 28 after operation, the rats were sacrificed,and the maxillary first molars on both sides of the rats were selected. HE staining and immunohistochemistry staining were used to observe and analyze the reparative dentin formation and the expression of dentin sialoprotein(DSP) in the odontoblasts. Results: The HE staining results showed that there were less inflammatory cells under the exposed pulp in that Matrilin-4 group at 3 d after operation compared with PBS group, and obvious vasodilation was found. At 7 d after operation, the inflammatory reaction under the exposed pulps in two groups was aggravated, but the inflammatory reaction in Matrilin-4 group was significantly lighter than that in PBS group, and the prophase dentin formation was found. After injury of 14 d,the pulp-exposed areas were covered by more complete dentin bridge in Matrilin-4 group; at 28 d after operation, compared with PBS group, there was a thick, complete and reparative dentin bridge under the exposed pulps, composed of tubular dentin in Matrilin-4 group, and there were reorganized odontoblastic layers beneath the dentin bridge. The results of immunohistochemistry showed that the positive expression strengths of DSP in the odontoblasts of the rats in two groups were gradually increased at 3, 7 and 14 d after operation, and were decreased at 28 d. The positive expression strengths of DSP in the odontoblast of the rats in Matrilin-4 group were stronger than those in PBS group at each time point after operation, and the positive expression strength reached the peak at 14 d. Conclusion: Compared with PBS group, the positive expression strength of DSP in the odontoblasts of the rats in Matrilin-4 group had significant differences (P<0.01) at 3, 7 and 14 d.

Key words: Matrilin-4, dental pulp injury repair, pulp capping agent, odontoblasts

CLC Number:

R781.3

XIE Jinfang, MENG Lin, GAO Huali, LI Xueyang, HU Xue, ZHANG Yingli, YIN Shuo. Promotion effect of Matrilin-4 on reparative dentin formation after dental pulp injury in rats[J].Journal of Jilin University(Medicine Edition), 2020, 46(01): 73-77.

References

[1] PARISAY I, GHODDUSI J, FORGHANI M. A review on vital pulp therapy in primary teeth[J]. Iran Endod J, 2015, 10(1):6-15.
[2] PARVIN M K, MORAL A A, SHIKDER Z H,et al. Evaluation of radiological outcomes of theracal light cured (TLC) and calcium hydroxide as indirect pulp capping agents in the treatment of deep carious lesion of permanent molar teeth[J]. Mymansingh Med J, 2018, 27(4):859-865.
[3] FRANSSON H. On the repair of the dentine barrier[J]. Swed Dent J Suppl, 2012, 226:9-84.
[4] ZAKARIAMN,CAHYANTOA,EL-GHANNAMA. Calcium release and physical properties of modified carbonate apatite cement as pulp capping agent in dental application[J]. Biomater Res, 2018, 22:35.
[5] PÄÄKKÖNEN V, VUORISTO J T, SALO T, et al. Comparative gene expression profile analysis between native human odontoblasts and pulp tissue[J]. Int Endod J, 2008, 41(2):117-127.
[6] CHEN C C, WEI X, LING J Q, et al. Expression of matrilin-2 and -4 in human dental pulps during dentin-pulp complex wound healing[J]. J Endod, 2011, 37(5):642-649.
[7] 谢金芳, 李晶, 李媛, 等. 细胞外基质Matrilin-4的研究进展[J]. 现代生物医学进展, 2017, 17(19):3795-3797.
[8] 杨楠, 李雯, 高华丽,等. Matrilin-2和Matrilin-4在大鼠牙髓损伤动物模型中的表达[J]. 现代口腔医学杂志, 2016,30(1):6-11.
[9] OPAL S, GARG S, DHINDSA A, et al. Minimally invasive clinical approach in indirect pulp therapy and healing of deep carious lesions[J]. J Clin Pediatr Dent, 2014, 38(3):185-192.
[10] HANADA K, MOROTOMI T, WASHIO A,et al.In vitro and in vivo effects of a novel bioactive glass-based cement used as a direct pulp capping agent[J]. J Biomed Mater Res Part B Appl Biomater, 2019, 107(1):161-168.
[11] LI Z F, CAO L H, FAN M W, et al. Direct pulp capping with calcium hydroxide or mineral trioxide aggregate:A meta-analysis[J]. J Endod, 2015, 41(9):1412-1417.
[12] LIKITPONGPIPAT N, SANGMANEEDET S, KLANRIT P, et al. Promotion of dental pulp wound healing in New Zealand white rabbits' teeth by thai propolis product[J]. J Vet Dent, 2019,36(1):17-24.
[13] SANGWAN P, SANGWAN A, DUHAN J, et al. Tertiary dentinogenesis with calcium hydroxide:A review of proposed mechanisms[J]. Int Endod J, 2013, 46(1):3-19.
[14] COLOMBO J S, MOORE A N, HARTGERINK J D, et al. Scaffolds to control inflammation and facilitate dental pulp regeneration[J]. J Endod, 2014, 40(4 Suppl):S6-12.
[15] TAHIRA T, JOUHAR R, GHANI H, et al. The effect of mineral trioxide aggregate as a direct pulp capping agent in permanent teeth[J]. Int J Oral Sci, 2018,10(6):310-313.
[16] BELOBROV I, PARASHOS P. Treatment of tooth discoloration after the use of white mineral trioxide aggregate[J]. J Endod, 2011, 37(7):1017-1020.
[17] ZHU L X, YANG J W, ZHANG J, et al. A comparative study of BioAggregate and ProRoot MTA on adhesion,migration, and attachment of human dental pulp cells[J]. J Endod, 2014, 40(8):1118-1123.
[18] LEAL F, DE-DEUS G, BRANDÃO C, et al. Similar sealability between bioceramic putty ready-to-use repair cement and white MTA[J]. Braz Dent J, 2013, 24(4):362-366.
[19] RAMOS J C, PALMA P J, NASCIMENTO R, et al. 1-year in vitro evaluation of tooth discoloration induced by 2 calcium silicate-based cements[J]. J Endod, 2016, 42(9):1403-1407.
[20] SHOKOUHINEJAD N, NEKOOFAR M H, PIRMOAZEN S, et al. Evaluation and comparison of occurrence of tooth discoloration after the application of various calcium silicate-based cements:An ex vivo study[J]. J Endod, 2016, 42(1):140-144.
[21] TIAN J, ZHANG YH, LAI ZH, et al. Ion release, microstructural, and biological properties of iroot BP plus and ProRoot MTA exposed to an acidic environment[J]. J Endod, 2017, 43(1):163-168.
[22] KIM J M, KANG S W, SHIN S M, et al. Inhibition of matrix metalloproteinases expression in human dental pulp cells by all-trans retinoic acid[J]. Int J Oral Sci, 2014, 6(3):150-153.

Promotion effect of Matrilin-4 on reparative dentin formation after dental pulp injury in rats

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Metrics

Comments

Recommended 10

[1]	HAN Yaolun, WANG Lu, MA Xin. Levels of serum melatonin in rats with pulpitis and its inhibitory effect on dental inflammation [J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1334-1339.
[2]	JIN Quan, XU Lishuo, HUANG Yu, JIAO Peng, CHEN Fei, CHE Hongze, MA Ning, ZHANG Li. Severe periodontitis complicated with oral lichen planus: A case report and literature review [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1298-1302.
[3]	CHE Hongze, CHE Yanhai, JIN Quan, XU Lishuo, MA Ning, ZHANG Li. Effect evaluation on application of gallnut extract in root canal therapy of patients with chronic periapical periodontitis [J]. Journal of Jilin University Medicine Edition, 2018, 44(01): 162-165.
[4]	LU Bing-ling,HUANG Xiao-jing,ZHANG Jin-xiu. Expression of MMP-8 in periapical tissues of rats with periapical periodontitis reinfected by Enterococcus faecalis [J]. Journal of Jilin University Medicine Edition, 2013, 39(3): 529-533.
[5]	. Advance research \|on non-international standard nickel-titanium \|rotary instruments in preparation of root canal [J]. J4, 2012, 38(3): 607-610.
[6]	LI Nan-nan,ZHANG Zhi-min\|WANG Cheng-kun,WANG Jin-rui,MENG Xiu-ping . Expression of \|MIP-1&alpha\|mRNA in \|inflammed \|pulp \|tissue and its significance [J]. J4, 2011, 37(2): 312-314.
[7]	LIU Ying\|LI Chun-yan\|SHAO Hai-feng\|LIANG Shuang. Analysis clinical effect of negative pressure \|drainage in treatment of acute periapical periodontitis [J]. J4, 2011, 37(2): 327-330.
[8]	JIANG Xin-peng，ZHANG Ying-li,LIN Chong-tao,OUYANG Hong-sheng. Effect of bFGF with different doses on proliferation of human dental pulp cells in vitro [J]. J4, 2008, 34(4): 633-635.
[9]	CHEN Jian-fang,HU Xiao-chun,ZHANG Ying-li. Expressions of matrix metalloproteinase 8 mRNA in apical granuloma and periapical cyst and their significances [J]. J4, 2008, 34(3): 466-468.
[10]	ZHANG Ying-li,HUANG Yang, ZHANG Ying-jie, ZHANG Tian-fu. Expression of TGF-β₁ on experimental periapical lesions in rats and its significance [J]. J4, 2006, 32(5): 794-796.
[11]	. Application of bFGF/collagen sustained release sponge in apexification [J]. J4, 2006, 32(3): 498-500.
[12]	. Progress research on reason of occurrence of apical microleakage and its detection methods [J]. Journal of Jilin University Medicine Edition, 2013, 39(6): 1307-1310.
[13]	GUO Ting-ting,WANG Cheng-kun. Evaluation on clinical efficiency of Path File NiTi files in mechanical glide path and preflaring of root cannal therapy [J]. Journal of Jilin University Medicine Edition, 2014, 40(02): 413-417.