半乳糖凝集素3表达抑制对人胃癌MGC-803细胞中Bcl-2和Bax表达的影响及其促凋亡作用

doi:10.13481/j.1671-587x.20200221

Abstract

Abstract: Objective: To investigate the influence of galectin-3 expression suppression in the apoptosis of human gastric cancer MGC-803 cells, and to provide a potential target for gene targeting therapy. Methods: The human gastric cancer MGC-803 cells were selected and divided into siRNA interference group, blank control group and negative control group. The MGC-803 cells in siRNA interference group were transfected with the siRNA which targeted galectin-3, the MGC-803 cells in blank control group were added only transfection reagent, while the MGC-803 cells in negative control group were transfected with negative siRNA. Flow cytometry and propidium iodide (PI) staining method were used to detect the apoptosis.Western blotting method was applied to determine the expressions of galectin-3,Bcl-2 and Bax proteins. Results: Compared with negative control group and blank control group, the expression level of galectin-3 protein in siRNA interference group was significantly decreased (P<0.05). The PI staining results showed that the number of apoptotic cells in siRNA interference group was increased significantly in which nuclear pyknosis, chromatin concentration, crescent shape and apoptotic bodies were found; meanwhile only a small amount of apoptotic cells in negative control group. The flow cytometry and Western blotting results showed that compared with negative control group and blank control group, the apoptotic rate of the MGC-803 cells in siRNA interference group was significantly increased(P<0.05), the expression level of Bcl-2 protein was decreased significantly (P<0.05),and the expression level of Bax protein had no significant difference(P>0.05). Conclusion: To suppress galectin-3 expression can promote the apoptosis of MGC-803 cells, suggesting that galectin-3 might play an oncogene role in the gastric cancer cells and has the possibility of becoming a target for targeting therapy.

Key words: galectin-3, MGC-803 cells, Bcl-2, Bax, flow cytometry

CLC Number:

R735.2

WU Xueyan, ZHAO Yina, WANG Xiaojie, MA Wenyi, WANG Xinjie, LI xin. Effect of galectin-3 expression suppression on expressions of Bcl-2 and Bax in gastric cancer MGC-803 cells and its promotion on apoptosis[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 335-339.

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Effect of galectin-3 expression suppression on expressions of Bcl-2 and Bax in gastric cancer MGC-803 cells and its promotion on apoptosis

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