白藜芦醇对大鼠视网膜缺血再灌注损伤后视网膜组织中Caspase-3和Bcl-2表达的影响

doi:10.13481/j.1671-587x.20180221

Abstract

Abstract: Objective:To study the effects of resveratrol on the expressions of Caspase-3 and Bcl-2 in the retina tissue of the rats with retinal ischemia-reperfusion injury (RIRI),and to investigate the therapeutic effect of resveratrol on the RIRI and its mechanism. Methods: A total of 90 SD rats were randomly divided into sham operation group,model group and treatment group,30 rats in each group.The RIRI models were established by pressing the anterior chamber of the rats.The rats in model and treatment groups received ischemia-reperfusion for 1,6,12,24,and 48h;the rats in treatment group were treated with micro-syringe intravitreal injection of 0.5 nmol·L^-1 of resveratrol 5 μL.The retinal tissue structure was observed by inverted microscope.The expression levels of Caspase-3 and Bcl-2 in retina tissue were detected by immunohistochemistry and Western blotting methods. Results: The retinal tissue edema of the rats in model group was found with vacuolar degeneration of the ganglion cells,and the arrangement of the cell layer was loose;the number of retinal ganglion cells was decreased,the boundary was blurred,and the nerve fiber layer thinned obviously.The degree of retinal tissue structure,the degree of injury and the degeneration of ganglion cells in treatment group were lighter than those in model group.The results of immunohistochemistry showed that the expressions of Caspase-3 and Bcl-2 in the retina tissue of the rats in treatment group were significantly increased compared with model group.The results of Western blotting method showed that the expression levels of Bcl-2 protein in the retina tissue of the rats in treatment group at different time points were increased compared with model group;and there were significant differences at 24 and 48 h (P<0.05);the expression levels of Caspase-3 protein in treatment group at different time points were lower than those in model group (P<0.05). Conclusion: Resveratrol can improve the retinal tissue structure of the RIRI rats,and its mechanism may be related to decreasing the expression level of Caspase-3 and increasing the expression level of Bcl-2 in the retinal tissue.

Key words: Bcl-2, resveratrol, retinal ischemia-reperfusion injury, Caspase-3

CLC Number:

R285.5

SU Chang, ZHOU Haisheng, GUO Shuling, SU Ruifeng, FU Xiaoxiao, TAN Xiaobo, LI Wei. Effects of resveratrol on expressions of Caspase-3 and Bcl-2 in retina tissue of rats with retinal ischemia-reperfusion injury[J].Journal of Jilin University Medicine Edition, 2018, 44(02): 321-326.

References

[1] Chen YJ,Huang YS,Chen JT,et al. Protective effects of glucosamine on oxidative-stress and ischemia reperfusion-induced retinal injury[J]. Invest Ophthalmol Vis Sci,2015,56(3):1506-1516.
[2] Fang IM,Yang CM,Yang CH.Chitosan oligosaccharides prevented retinal ischemia and reperfusion injury via reduced oxidative stress and inflammation in rats[J].Exp Eye Res,2015,130(7):38-50.
[3] 贾茜钰,刘勤.中药提取物对视网膜缺血再灌注损伤防治作用的研究进展[J].国际眼科杂志,2015,15(5):810-812.
[4] de la Lastra CA,Villegas I.Resveratrol as an antioxidant and pro-oxidant agent:mechanisms and clinical implications[J].Biochem Soc Trans,2007,35(Pt5):1156-1160.
[5] Ban JY,Cho SO,Choi SH,et al.Neuroprotective effect of Smilacis chinae rhizome on NMDA-induced neurotoxicity in vitro and focal cerebral ischemia in vivo[J].J Pharmacol Sci,2008,106(1):68-77.
[6] 陈梦微,邓群仙,张金容,等.葡萄不同品种和组织中白藜芦醇含量及其抗氧化活性分析[J].食品与机械,2017,33(1):150-154.
[7] 王娜,李峰,邢晓玉,等.低毒白藜芦醇脂质体的制备及其抗氧化活性研究[J].广州化工,2017,45(3):37-38.
[8] 王浩,刘索新,鞠学红.视网膜缺血再灌注模型的建立与观察研究进展[J].国际眼科杂志,2012,12(10):1902-1903.
[9] Wang S,Ye Q,Tu J,et al.Curcumin protects against hypertension aggravated retinal ischemia/reperfusion in a rat stroke model[J].Clin Exp Hypertens,2017,39(8):711-717.
[10] Kal A,Kal O,Akillioglu I,et al.The protective effect of prophylactic ozone administration against retinal ischemia-reperfusion injury[J].Cutan Ocul Toxicol,2017,36(1):39-47.
[11] Sakamoto K,Suzuki Y,Kurauchi Y,et al.Hydrogen sulfide attenuates NMDA-induced neuronal injury via its anti-oxidative activity in the rat retina[J].Exp Eye Res,2014,120(4):90-96.
[12] Li X,Kang Q,Gao S,et al.Transplantation with retinal progenitor cells repairs visual function in rats with retinal ischemia-reperfusion injury[J].Neurosci Lett,2014,558:8-13.
[13] Chen L,Qi Y,Yang X.Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina[J].Ophthalmic Res,2015,54(3):157-68.
[14] Chen Q,Wang H,Liao S,et al.Nerve growth factor protects retinal ganglion cells against injury induced by retinal ischemia-reperfusion in rats[J].Growth Factors,2015,33(2):149-159.
[15] Wang J,Sun Z,Shen J,et al.Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-κB[J].Oxid Med Cell Longev,2015,2015:970156.
[16] Xu J,Zhou M,Ouyang J,et al.Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells[J].Chin JCancer Res,2013,25(2):183-191.
[17] Zarch AV,Toroudi HP,Soleimani M,et al.Neuroprotective effects of diazoxide and its antagonism by glibenclamide in pyramidal neurons of rat hippocampus subjected to ischemia-reperfusion-induced injury[J].Int J Neurosci,2009,119(9):1346-1361.
[18] 刘莹,张花治,白丽君,等.原花青素对视网膜缺血再灌注损伤大鼠视网膜结构及核转录因子-κB表达的影响[J].中国中医药信息杂志,2013,20(5):32-34.
[19] Choudhuri S,Chowdhury IH,Das S,et al.Role of NF-κB activation and VEGF gene polymorphisms in VEGF up regulation in non-proliferative and proliferative diabetic retinopathy[J].Mol Cell Biochem,2015,405(1/2):265-279.
[20] Joachim SC,Jehle T,Boehm N,et al. Effect of ischemia duration on autoantibody response in rats undergoing retinal ischemia-reperfusion[J].Ophthalmic Res,2012,48(2):67-74.
[21] Ishikawa S,Hirata A,Nakabayashi J,et al.Neuroprotective effect of small interfering RNA targeted to caspase-3 on rat retinal ganglion cell loss induced by ischemia and reperfusion injury[J].Curr Eye Res,2012,37(10):907-913.
[22] Kerr NM,Johnson CS,Zhang J,et al.High pressure-induced retinal ischaemia reperfusion causes upregulation of gap junction protein connexin43 prior to retinal ganglion cell loss[J].Exp Neurol,2012,234(1):144-152.
[23] Singh M,Savitz SI,Hoque R,et al.Cell-specific caspase expression by different neuronal phenotypes in transient retinal ischemia[J].J Neurochem,2001,77(2):466-475.

Effects of resveratrol on expressions of Caspase-3 and Bcl-2 in retina tissue of rats with retinal ischemia-reperfusion injury

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