Journal of Jilin University(Medicine Edition) ›› 2020, Vol. 46 ›› Issue (6): 1274-1282.doi: 10.13481/j.1671-587x.20200626

• Research in clinical medicine • Previous Articles     Next Articles

Detection of serum levels of gastric cancer-related cytokines and their clinical significances in diagnosis of gastric cancer

Ruiying SUN1,2,Yang LI1,Xu ZHAO3,Linlin QU2,Wei XU2(),Yunli ZHANG1()   

  1. 1.Departement of Clinical Laboratory,First Affiliated Hospital,Jinzhou Medical University,Jinzhou 121000,China
    2.Department of Clinical Laboratory,First Hospital,Jilin University,Changchun 130021,China
    3.Department of Hepatobiliary and Pancreatic Medicine,First Hospital,Jilin University,Changchun 130021,China
  • Received:2020-04-23 Online:2020-11-28 Published:2022-08-24
  • Contact: Wei XU,Yunli ZHANG E-mail:xuwei0210@sina.com;ZYL8546@126.com

Abstract: Objective

To detect the expression levels of 17 kinds of gastric cancer-related cytokines in serum of the gastric cancer patients and study their correlations with miR-10b level, and to screen the tumor markers which were meaningful for the diagnosis of gastric cancer and to explore their application values in diagnosis of gastric cancer.

Methods

Based on the case-control study design, 34 cases of serum specimens from patients with definite histopathological diagnosis of gastric adenocarcinoma were selected as gastric cancer group, and 80 cases of serum specimens from healthy people who passed the physical examination were selected as control group. Luminex detection technology was used to detect the levels of gastric cancer-related cytokines in serum samples of the subjects in two groups. The tumor markers that were meaningful for the diagnosis of gastric cancer were screened from the following indexes such as macrophage inflammatory protein-3α (CCL20), interleukin-2 (IL-2), interleukin-4 (IL-4), Interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-17A (IL-17A), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), monocyte chemoattractant protein-4 (CCL13), interferon-induced T cell alpha chemokine (CXCL11), B-lymphocyte chemoattractant (CXCL13), bone morphogenetic protein 4 (BMP4), bone morphogenetic protein7 (BMP7), hepatocyte growth factor (HGF), granulocyte-macrophage colony stimulating factor (GM-CSF), platelet derived growth factor-BB (PDGF-BB).Quantitative real-time polymerase chain reaction(qRT-PCR) method was used to detect the serum miR-10b level of the patients in gastric cancer group;electrochemiluminescence method was used to detect the serum tumor markers carcinoembryonic antigen(CEA), carbohydrate antigen 19-9(CA19-9) and carbohydrate antigen 72-4(CA72-4) levels of the patients in gastric cancer group.

Results

Compared with control group, the serum levels of CCL20, IL-2, IL-4, IL-6, IL-17A, TNF-α, CCL13, BMP-4, and HGF of the patients in gastric cancer group were significantly increased (P<0.05); the Logistic regression analysis results showed that the serum levels of CCL20, IL-2, IL-4, IL-6, IL-17A, TNF-α, CXCL11, CCL13, BMP-4, BMP-7, and HGF were associated with the risk of gastric cancer ( P<0.05); the receptor operation characteristic curve(ROC) analysis results showed that the area under the curve (AUC) of CCL20 in diagnosis of gastric cancer was significantly better than combinnation diagnosis of the three tumor markers CEA, CA19-9,and CA72-4; When the cut-off value of CCL20 was 6.71 ng·L-1, the diagnostic specificity, sensitivity, positive predictive value, negative predictive value, positive likelihood ratio, negative predictive ratio, positive likelihood ratio, negative predictive ratio and diagnostic accuracy were 76.5%, 91.3%, 78.8%, 90.1%, 8.74, 0.26,and 86.84%, respectively. The Spearman correlation analysis results showed that 17 kind of cytokines levels had poor correlations with miR-10b level, and the level of TNF-α had a certain correlation with miR-10b level(r=0.335, P=0.075).

Conclusion

When 6.71 ng·L-1 is used as the diagnostic cut-off value of the serum cytokine CCL20, its diagnostic sensitivity is significantly better than the tumor markers CEA, CA19-9 and CA72-4 commonly used in clinic, and the specificity is also higher.

Key words: stomach neoplasms, cytokine, miR-10b, macrophage inflammatory protein -3α, tumor marker

CLC Number: 

  • R735.2