Abstract: Abstract:Objective To analyze the genotypes of clinical isolates of Mycobacterium tuberculosis in Jilin Province with multiple locus variable numbers of tandem repeats（MLVA）genotyping.Methods Mycobacterium tuberculosis strains were isolated from patient samples in Jilin Province.Genomic DNA was extracted and 12 variable numbers of tandem repeats(VNTR) loci were amplified.The gels were analyzed by Totalab  software and the results were clustered with BioNumerics(6.0).Results Analysis of a total of 110 isolates with the 12 VNTR loci showed great genetic polymorphism in these isolates.Nine of the 12 loci showed the Hunter-Gaston index(HGI) were higher than 0.5.The clustering results showed that total of 6 genotype groups could be defined,of which group 5 was the most represent one,accounting for 34％ of the isolates.The less represented genotypes included group 1,accounting for 15％.Strains of group 5 mainly distributed in Tonghua and Songyuan.Conclusion Mycobacterium tuberculosis isolates in Jilin Province shows great genetic polymorphisms.Different cities have different genotype profiles.

Key words: Mycobacterium tuberculosis, genoty
ping, multiple 1ocus variable numbers of tandem repeats analysis, Hunter-Gaston index